Evidence mapPaperPMID 42215531Full record

ArticleScientific reports2026

Downregulated m⁵C regulator NSUN6 enhances proliferation, migration and affects immune regulation in ovarian cancer.

Kuan Hu, Wenjing Shi, Zongjiang Zhou, Xinyue Deng, Juanni Li

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In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Kuan Hu *Department of Hepatobiliary Surgery, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Wenjing Shi *Department of Pathology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Zongjiang ZhouDepartment of Pathology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Xinyue DengDepartment of Pathology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Juanni LiDepartment of Pathology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China. lijuanni2014@csu.edu.cn.

Funding

National Natural Science Foundation of China 82103300Natural Science Foundation of Hunan Province 2023JJ30899
6 · The paper itself

Abstract

Ovarian cancer (OC) remains one of the most lethal gynecological malignancies, largely due to late diagnosis and high metastatic potential. RNA 5-methylcytosine (m⁵C) modification has emerged as a key regulatory mechanism in cancer biology, yet the role of m⁵C-related genes such as NSUN6 in OC remains poorly understood. In this study, we first obtained three OC datasets and one m5C-related dataset from the GEO database and identified differentially expressed genes (co-DEGs) using Venn diagram analysis. Kaplan Meier plotter showed that NSUN6 was the only regulator significantly associated with patient prognosis. Additionly, Western Blot, Online database analysis and immunohistochemical staining evaluation showed that NSUN6 was significantly downregulated in OC and negatively correlated with tumor stage, metastasis and survival. Next, we overexpressed NSUN6 in OC cell lines. CCK8, wound healing and Transwell assays demonstrated that NSUN6 overexpression inhibited OC cell proliferation, migration, and invasion. Rescue experiments confirmed NSUN6-mediated suppression of AKT phosphorylation. In vivo xenograft models confirmed the tumor-suppressive effect of NSUN6, showing reduced tumor burden in both intraperitoneal and subcutaneous models. Bioinformatic analyses revealed enrichment in immune-related pathways and correlations with immune infiltration markers, which were further validated using ovarian cancer clinical samples. Collectively, our results demonstrate that NSUN6 is downregulated in OC and suppresses OC proliferation, migration, and invasion by inhibiting AKT activity, highlighting its potential as a therapeutic target in OC.

Indexed as

MethyltransferasesOvarian Neoplasms5-MethylcytosineAnimalsCell Line, TumorCell MovementCell ProliferationDown-RegulationFemaleGene Expression Regulation, NeoplasticHumansMiceMice, NudePrognosisProto-Oncogene Proteins c-aktRNA Methylation5-MethylcytosineMethyltransferasesProto-Oncogene Proteins c-aktM⁵CMetastasisNSUN6OCP-AKT

Identifiers

PMID42215531
PMCPMC13454582

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.