ArticlePloS one2026
Establishment and application of a quadruple target real-time PCR assay for detecting Yersinia pestis.
Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to establish an efficient method for high-throughput detection of Yersinia pestis (Y. pestis), a quadruple target real-time polymerase chain reaction assay was developed based on the specific target genes of Y. pestis (caf1, pla, ymt, and ypo-1094). Its sensitivity, specificity, and repeatability were evaluated, and clinical serum samples were tested by the established method. The results showed that no cross-reactivity was observed with other bacterial nucleic acids. The optimal linear detection range for caf1, pla, ymt, and ypo-1094 was 12.09 × 10-⁶-12.09 × 101 ng/μL, and the lower limit of detection was 12.09 × 10-4 ng/μL. Four different DNA concentrations of caf1, pla, ymt, and ypo-1094 (10-4, 10-3, 10-2, 10-1and 101 ng/μL) were tested five times, achieving good repeatability. In the clinical sample detection, all the Y. pestis - positive samples were identified. The established method has potential for clinical use for rapid detection of Y. pestis with high specificity and high sensitivity.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.