Evidence map›Paper›PMID 42226245›Full record

ArticleBiology direct2026

Pyrroloquinoline quinone protects heat-stressed porcine oocytes and improves early embryonic development via maintaining mitochondrial integrity and redox homeostasis.

Mianqun Zhang, Qimei Xu, Bo Jia, Xue Zhang, Khienduc Lay, Mengchan Li, Yan Zou, Ziyi Wang, Xu Peng, Yang Gao and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology direct, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Mianqun Zhang *Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, P. R. China.
Qimei Xu *Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, P. R. China.
Bo Jia *Key Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, P. R. China.
Xue Zhang *Guangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hong Kong Metabolism & Reproduction Joint Laboratory, Reproductive Medicine Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou, 510317, China.
Khienduc LayKey Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, P. R. China.
Mengchan LiKey Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, P. R. China.
Yan ZouSchool of Biology and Food Engineering, Hefei Normal University, Hefei, 230061, P. R. China.
Ziyi WangSchool of Biology and Food Engineering, Hefei Normal University, Hefei, 230061, P. R. China.
Xu PengSchool of Biology and Food Engineering, Hefei Normal University, Hefei, 230061, P. R. China.
Yang GaoSchool of Biology and Food Engineering, Hefei Normal University, Hefei, 230061, P. R. China.
Changyin ZhouGuangzhou Key Laboratory of Metabolic Diseases and Reproductive Health, Guangdong-Hong Kong Metabolism & Reproduction Joint Laboratory, Reproductive Medicine Center, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou, 510317, China. zhoucy@gd2h.org.cn.
Yanfeng XueKey Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, P. R. China. xueyanfeng1990@163.com.
Yunhai ZhangKey Laboratory of Local Livestock and Poultry Genetical Resource Conservation and Breeding of Anhui Province, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, P. R. China. dkzyh@ahau.edu.cn.

Funding

National Key Research and Development Program of China 2023YFD13000502National Natural Science Foundation of China 32272881Natural Science Foundation of Anhui Province 2308085QC82
6 · The paper itself

Abstract

Heat stress (HS) is a major environmental factor that impairs female fertility by disrupting oocyte quality and developmental competence. Pyrroloquinoline quinone (PQQ), a redox cofactor with strong antioxidant and mitochondrial-protective properties, has recently attracted attention for its cytoprotective roles in mammalian cells. Here, we investigated whether PQQ supplementation could alleviate HS-induced porcine oocyte damage and improve subsequent embryonic development. Our results showed that PQQ treatment significantly restored the rates of germinal vesicle breakdown (GVBD) and first polar body extrusion (PBE) in heat-stressed oocytes. PQQ markedly reduced intracellular reactive oxygen species (ROS) accumulation, enhanced mitochondrial membrane potential (ΔΨm), and maintained ATP levels, indicating improved mitochondrial function. Furthermore, PQQ attenuated DNA damage (γH2A.X foci) and apoptosis (Caspase-3 activation), preserved spindle morphology and actin distribution, and normalized cortical granule and ovastacin localization, suggesting improved cytoplasmic maturation. Importantly, embryos derived from PQQ-treated oocytes exhibited higher cleavage and blastocyst formation rates compared with the HS group. Collectively, these findings demonstrate that PQQ effectively mitigates HS-induced oocyte dysfunction by maintaining mitochondrial integrity, redox balance, and cytoskeletal organization, thereby improving oocyte competence and early embryonic development under heat stress.

Indexed as

Embryonic DevelopmentHeat-Shock ResponseMitochondriaOocytesPQQ CofactorAnimalsFemaleHomeostasisMembrane Potential, MitochondrialOxidation-ReductionReactive Oxygen SpeciesSwinePQQ CofactorReactive Oxygen SpeciesEmbryonic developmentHeat stressMitochondrial functionOxidative stressPorcine oocytePyrroloquinoline quinone

Identifiers

PMID42226245
PMCPMC13435474

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.