ArticlebioRxiv : the preprint server for biology2026
A RHNO1-ATR/Chk1 positive feedback loop sustains the DNA replication stress response.
Niphat Jirapongwattana, Carley M Conover, Catalina Trujillo Jaramillo, Kishore K Chiruvella, Joonyoung Her, Gargi Ghosal, Samuel F Bunting, Dale A Ramsden, Adam R Karpf
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In one paragraphArticle in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
9 authors.
Niphat JirapongwattanaEppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha, NE, 68198-6805.ORCID 0000-0003-4720-4097 Carley M ConoverEppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha, NE, 68198-6805.
Catalina Trujillo JaramilloEppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha, NE, 68198-6805.
Kishore K ChiruvellaDepartment of Biochemistry and Biophysics and Curriculum in Genetics and Molecular Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC.
Joonyoung HerDepartment of Molecular Biology and Biochemistry, Rutgers, the State University of New Jersey, Piscataway, NJ, 08854-8021.
Gargi GhosalFred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, 68198-6805.
Samuel F BuntingDepartment of Molecular Biology and Biochemistry, Rutgers, the State University of New Jersey, Piscataway, NJ, 08854-8021.
Dale A RamsdenDepartment of Biochemistry and Biophysics and Curriculum in Genetics and Molecular Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC.
Adam R KarpfEppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha, NE, 68198-6805.ORCID 0000-0002-0866-0666 Funding
UNMC/EPPLEY CANCER CENTER SUPPORT GRANTP30CA036727 · NCI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI James Eudy · 1985 to 2026
$55.0MStructural determinants of Pol theta functionP01CA247773 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI DALE A RAMSDEN · 2020 to 2026
$15.2MProject 4: The BRCA Network in Medulloblastoma Responses to Replication StressP01CA250957 · NCI · RUTGERS BIOMEDICAL AND HEALTH SCIENCES · PI DE, SUBHAJYOTI · 2021 to 2025
$11.3MMechanisms underlying USP1-mediated bypass of EWS-FLI1 oncogene-induced replication stress in Ewing sarcomaR01CA263504 · NCI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI GARGI GHOSAL · 2022 to 2026
$1.7MRegulation of SPRTN protease and SPRTN-mediated DNA-Protein Crosslink RepairR01GM141232 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI GHOSAL, GARGI · 2022 to 2025
$1.3MTargeting RHNO1 in Ovarian CancerR21CA273399 · NCI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI KARPF, ADAM R. · 2023 to 2024
$384kNCI NIH HHS P01 CA247773NCI NIH HHS P01 CA250957NCI NIH HHS P30 CA036727NCI NIH HHS R01 CA263504NCI NIH HHS R21 CA273399NIGMS NIH HHS R01 GM141232
6 · The paper itselfAbstract
Elevated DNA replication stress is a common feature of cancer cells, rendering them dependent on ATR/Chk1 signaling, which controls the DNA replication stress checkpoint, for survival. Although the activation of ATR/Chk1 signaling is well-established, how this signal is maintained until replication stress is fully resolved is less understood. Here, we investigated the roles of RAD9-HUS1-RAD1 interacting nuclear orphan 1 (RHNO1) in cancer progression and its involvement in maintaining ATR/Chk1 signaling during the DNA replication stress response. We demonstrate that depletion of RHNO1 significantly inhibits cancer cell proliferation
Identifiers
PMID42239230
PMCPMC13228601
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