ArticleResearch square2026
Alternative 3' Polyadenylation Responses to Acute Ethanol Exposure Differ Between Drosophila Populations.
Article in Research square, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Alternative polyadenylation is a pervasive post-transcriptional mechanism that generates mRNA isoforms with distinct 3' untranslated regions (3' UTRs), which can influence transcript stability, localization and translation. Ethanol is an environmental stressor for Drosophila melanogaster, and natural populations show distinct responses to ethanol exposure, suggesting that the genetic background can modulate phenotypic response. Yet, how the natural genetic variation modulates ethanol-responsive APA remains understudied. We profiled 3' end isoforms following acute ethanol exposure in three French (cosmopolitan) and three Zambian (ancestral range) D. melanogaster genotypes and identified numerous significant APA events across all genotypes. Ethanol-induced APA was widespread but highly genotype - and population - dependent: the French genotypes showed an enrichment of proximal shifts (3' UTR shortening), while Zambian genotypes were biased toward distal shifts (3' UTR lengthening). Most of the ethanol-responsive APA events were private to a single population, with relatively few genes exhibiting conserved directional changes across both backgrounds. We also classify genes showing opposite-direction shifts between populations, and others with opposite-direction shifts between various classes of APA. For two-site events, inter-poly(A) site distance did not predict response direction; however, spacing strongly predicted the magnitude of remodeling, with larger separations enabling greater absolute 3' UTR length changes. Our findings support a model in which ethanol-responsive 3' end regulation is a flexible genetically contingent, and that population-specific APA remodeling represent a plausible molecular substrate contributing to adaptation.
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.