Evidence map›Paper›PMID 42244699›Full record

ArticlebioRxiv : the preprint server for biology2026

Fully Modified SpyCas9 Guide RNAs Enable Robust Genome Editing In Cells and In Vivo.

Kim Anh Vu, Han Zhang, Nadia Amrani, Gitali Devi, Nicholas Gaston, Jonathan Lee, Stacy A Maitland, Zexiang Chen, Dimas Echeverria, Pengpeng Liu and 17 more

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

27 authors.

Kim Anh VuRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0002-6030-567X
Han ZhangRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0003-1090-2967
Nadia AmraniRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0001-6871-7962
Gitali DeviRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0002-9080-4656
Nicholas GastonRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0002-3862-9002
Jonathan LeeRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Stacy A MaitlandDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Zexiang ChenRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Dimas EcheverriaRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Pengpeng LiuDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0002-4891-8780
Karthikeyan PonnienselvanDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0002-8016-9433
Matthew B HanlonDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Connor LucasRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Kevin LukDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0002-9727-1960
Jacquelyn SousaRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
David CooperRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Alyxandr SrnkaRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Julia M Rembetsy-BrownRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Aditya Valji AnsodariaRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0003-1408-1182
Nathan BamideleRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0001-9051-5531
Aamir MirRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0003-0144-3914
Ken YamadaRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0002-5714-8298
Julia F AltermanRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0002-6195-0857
Anastasia KhvorovaRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0001-6928-8071
Scot A WolfeDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0002-7042-201X
Erik J SontheimerRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0002-0881-0310
Jonathan K WattsRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.ORCID 0000-0001-5706-1734

Funding

The CRISPR Vision Program: Nonviral Genome Editing Platforms to Treat Inherited Retinal Channelopathies Epqt SupplementU19NS132296 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI Krishanu Saha · 2023 to 2026
$36.8M
Precision Editing of Granulin Mutations that Cause Frontotemporal DementiaU01NS145218 · NINDS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI GAO, FEN-BIAO, SONTHEIMER, ERIK J. · 2025 to 2025
$6.6M
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and DonorsUH3TR002668 · NCATS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI KHVOROVA, ANASTASIA, SONTHEIMER, ERIK J. · 2021 to 2022
$3.1M
Mid-Scale RNA Synthesis, Purification and Quality Control SystemS10OD020012 · OD · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI KHVOROVA, ANASTASIA · 2015 to 2015
$563k
NCATS NIH HHS UH3 TR002668NIH HHS S10 OD020012NINDS NIH HHS U01 NS145218NINDS NIH HHS U19 NS132296
6 · The paper itself

Abstract

Precision engineering of CRISPR/Cas9 components has advanced genome editing toward therapeutic applications. Completely chemically stabilized guide RNAs (gRNAs) have the potential to improve

Identifiers

PMID42244699
PMCPMC13232067

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.