Optimization of Small-Molecule Wnt Pathway Activation with CHIR99021 for Human Induced Pluripotent Stem Cell Differentiation to Cardiomyocytes.

Bhavya Tikaria, Aaron H Wasserman, Bana Abolibdeh, Charles C Hong

Abstract read

In one paragraph

Article in The National high school journal of science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Bhavya Tikaria12th Grade, Okemos High School, Okemos, MI, United States.

Aaron H WassermanDepartment of Medicine, Michigan State University College of Human Medicine, East Lansing, MI, USA.

Bana AbolibdehDepartment of Medicine, Michigan State University College of Human Medicine, East Lansing, MI, USA.

Charles C HongDepartment of Medicine, Michigan State University College of Human Medicine, East Lansing, MI, USA.

Funding

Mechanistic Insights into The Role of Microtubule Organizing Centers on Cardiomyocyte Structure and FunctionR01HL167133 · NHLBI · UNIVERSITY OF MARYLAND BALTIMORE · PI CHARLES C HONG · 2023 to 2026

$2.0M

NHLBI NIH HHS R01 HL167133

6 · The paper itself

Abstract

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are a promising tool to model and investigate human heart disease in vitro. To achieve this goal, differentiation protocols must be optimized to produce pure populations of somatic cells, such as cardiomyocytes (CMs). CHIR99021 (CHIR) is a small molecule that inhibits the enzyme glycogen synthase kinase-3 beta (GSK-3

Indexed as

Cardiomyocytes (CMs)Cardiovascular diseaseCentrosomeCHIR99021 (CHIR)DifferentiationHuman induced pluripotent stem cells (hiPSCs)Rotatin (RTTN)Wnt pathway

Identifiers

PMID42245385

PMCPMC13231619

What Socratic holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.