Evidence mapPaperPMID 42260918Full record

ArticleBrain and behavior2026

Electroacupuncture Ameliorates Cognitive Impairment in Vascular Dementia Rats: Potential Involvement of the Astrocyte-Synapse Axis.

Sijing Guo, Qian Liu, Shihao Lin, Taotao Zhou, Qin Hu, Zhongsheng Tang

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Article in Brain and behavior, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Sijing GuoSchool of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang, China.
Qian LiuSchool of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang, China.
Shihao LinDepartment of Neurosurgery, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Taotao ZhouSchool of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang, China.
Qin HuDepartment of Neurosurgery, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Zhongsheng TangSchool of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang, China.

Funding

National Natural Science Foundation of China 82360873Science and Technology Plan Project of Guizhou Province ZD(2025)016
6 · The paper itself

Abstract

introductionThis study investigated whether electroacupuncture at "Zhisanzhen" (EA-ZSZ) alleviates cognitive impairment in vascular dementia (VD) rats by regulating the astrocyte-synapse axis, reshaping the hippocampal secretory microenvironment, and preserving synaptic structural integrity.

methodsSprague-Dawley rats were divided into sham, VD, VD+EA, and VD+Nim (nimodipine) groups. The VD model was established via a modified bilateral common carotid artery occlusion (2-VO). After 21 days, learning and memory were evaluated using the Morris water maze. Hippocampal CA1 histopathology and ultrastructure were assessed by H&E and transmission electron microscopy (TEM). RNA-seq identified differentially expressed genes (DEGs) and pathways. GFAP, BDNF, bFGF, and cytokines (IL-1β, IL-6, and TNF-α) were measured via immunofluorescence, Western blot, and ELISA.

resultsCompared to sham group, VD rats exhibited cognitive impairment, neuronal disorganization, and synaptic disruption. EA-ZSZ markedly alleviated cognitive deficits, outperforming nimodipine in spatial learning/memory, and restored synaptic ultrastructure (showing clearer clefts and increased vesicle abundance on TEM). RNA-seq showed that EA-ZSZ normalized pathways associated with cytokine-cytokine receptor interactions and synaptic signaling, restoring key "reversal genes" (Mdk, Homer1, and Npas4). These changes were accompanied by reduced GFAP, upregulated hippocampal BDNF/bFGF, and suppressed systemic cytokines (IL-1β, IL-6, and TNF-α).

conclusionEA-ZSZ exerts significant neuroprotective effects in VD rats by modulating the astrocyte-synapse axis, suppressing neuroinflammation, and enhancing neurotrophic/structural support within the hippocampal microenvironment. This highlights its multi-target advantage over monotherapies in repairing damaged synaptic architecture, supporting its clinical use.

Indexed as

AstrocytesCognitive DysfunctionDementia, VascularElectroacupunctureSynapsesAnimalsDisease Models, AnimalHippocampusMaleMaze LearningRatsRats, Sprague-Dawleyastrocyte–synapse axiselectroacupunctureneuroinflammationsynaptic structural integrityvascular dementia

Identifiers

PMID42260918
PMCPMC13247131

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.