Evidence map›Paper›PMID 42268713›Full record

ArticleCell reports2026

The MLLT3 YEATS domain binds histone marks (H3K9/18/27ac/cr) and ncRNA (7SK), linking transcription and RNA signaling to regulate hematopoiesis.

Adam Boulton, Ashish Kabra, Nicholas Achille, Emmalee R Adelman, Dimitrios G Anastasakis, Felipe Beckedorff, Pradeep Kumar Reddy Cingaram, Devin G Roller, Ying Zhang, Benjamin Leach and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors.

Adam BoultonDepartment of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22903, USA.
Ashish KabraDepartment of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22903, USA.
Nicholas AchilleDepartment of Cancer Biology, Loyola University Chicago, Maywood, IL 60153, USA.
Emmalee R AdelmanDepartment of Biochemistry, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Dimitrios G AnastasakisRNA Molecular Biology Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Intramural Research Program, National Institutes of Health, Bethesda, MD 20892, USA.
Felipe BeckedorffSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Department of Human Genetics, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Pradeep Kumar Reddy CingaramSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Department of Human Genetics, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Devin G RollerDepartment of Urology, Charlottesville, VA, USA.
Ying ZhangDepartment of Microbiology, Immunology and Cancer Biology, University of Virginia, Charlottesville, VA 22903, USA.
Benjamin LeachDepartment of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22903, USA.
Daniel GioeliDepartment of Urology, University of Virginia, and University of Virginia NCI-designated Comprehensive Cancer Center, Charlottesville, VA 22903, USA; University of Virginia NCI-designated Comprehensive Cancer Center, Charlottesville, VA 22903, USA.
Ramin ShiekhattarSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Department of Human Genetics, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Akihiko YokoyamaTsuruoka Metabolomics Laboratory, National Cancer Center, Tsuruoka, Yamagata, Japan; Division of Translational Genomics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Chiba, Japan.
Markus HafnerRNA Molecular Biology Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Intramural Research Program, National Institutes of Health, Bethesda, MD 20892, USA.
Maria FigueroaDepartment of Biochemistry, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Nancy Zeleznik-LeDepartment of Cancer Biology, Loyola University Chicago, Maywood, IL 60153, USA.
John H BushwellerDepartment of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22903, USA; Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA; University of Virginia NCI-designated Comprehensive Cancer Center, Charlottesville, VA 22903, USA. Electronic address: jhb4v@virginia.edu.

Funding

AF9(MLLT3) Function in Leukemia and Normal HematopoiesisR01CA233749 · NCI · UNIVERSITY OF VIRGINIA · PI BUSHWELLER, JOHN HACKETT, FIGUEROA, MARIA EUGENIA · 2019 to 2023
$3.2M
NCI NIH HHS R01 CA233749
6 · The paper itself

Abstract

MLLT3 (AF9) is a critical regulator of hematopoiesis. The YEATS domain of MLLT3 binds to acylated histones (H3K9ac/cr). We showed that the YEATS domain also binds to RNA. PAR-CLIP data demonstrated binding to the snRNA 7SK. Biochemical studies show high-affinity binding to stem loop 4 (SL4) of 7SK. NMR chemical shift perturbation mapping of the binding site for 7SK SL4 on the YEATS domain was used to generate a model of the complex. Based on the model, mutations were identified that disrupt 7SK binding without affecting H3K9ac binding. Introduction of these mutations into full-length MLLT3 skewed hematopoiesis toward the lymphoid lineage, abrogated the transforming properties of NES-AF9, and reduced the self-association of MLLT3. PRO-Seq and RNA-Seq identified genes driving the observed phenotype and showed a block in transcriptional initiation with the loss of RNA binding. Our results demonstrate a critical role for RNA binding in the function of MLLT3.

Indexed as

HematopoiesisHistone CodeHistonesNuclear ProteinsRNA, Small NuclearRNA, UntranslatedTranscription, GeneticAnimalsBinding SitesHumansProtein BindingProtein DomainsSignal TransductionHistonesMLLT3 protein, humanNuclear ProteinsRNA, Small NuclearRNA, Untranslated7SKAF9CP: molecular biologyhematopoiesisMLLT3YEATS domain

Identifiers

PMID42268713
PMCPMC13383808

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.