Improved chromosome-level genome assembly of Pleurotus ostreatus CCMSSC 00389.

Yanjiao Ma, Chenyang Huang, Mengran Zhao, Zeyin Wu, Fangjie Yao, Lijiao Zhang

Abstract read

In one paragraph

Article in G3 (Bethesda, Md.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Yanjiao MaCollege of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin 130118, China.ORCID 0009-0000-0315-7458

Chenyang HuangState Key Laboratory of Efficient Utilization of Arable Land in China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China.

Mengran ZhaoState Key Laboratory of Efficient Utilization of Arable Land in China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China.

Zeyin WuCollege of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin 130118, China.

Fangjie YaoCollege of Horticulture, Jilin Agricultural University, 2888 Xincheng Street, Changchun, Jilin 130118, China.

Lijiao ZhangState Key Laboratory of Efficient Utilization of Arable Land in China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, China.ORCID 0009-0005-1699-805X

Funding

China Agriculture Research System (CARS20)Fundamental Research Funds for Central Non-profit Scientific Institution G2026-01-91National Key Research and Development Program of China 2022YFD1200600National Natural Science Foundation of China 32302638

6 · The paper itself

Abstract

Pleurotus ostreatus is one of the major cultivated edible mushrooms in China, and possesses high nutritional value and prominent medicinal properties. A chromosome-level genome assembly of this species is therefore of great significance for both basic biological research and industrial applications. In this study, we present a chromosome-level genome assembly of the commercial P. ostreatus cultivar CCMSSC 00389, which shows improved continuity and completeness relative to previously published genome assemblies. The new assembly genome consists of 12 pseudo-chromosomes with a total length of 40.39 Mb. Compared with previous versions, the total genome size increased by 11.31%, the number of scaffolds was reduced from 2,529 to 20, and the scaffold N50 length was improved 9.53 fold. Accordingly, the average gene length increased from 1,523 to 1,867.5 bp, and the number of genes functionally annotated in public databases rose from 11,095 to 12,514. BUSCO analysis was performed to evaluate the integrity and completeness of the newly assembled genome. A total of 269 complete BUSCO genes were identified, accounting for 92.76% of the total assessed genes, demonstrating the acceptable completeness of the new assembled genome. This high-quality genome provides a crucial foundation for exploring evolutionary dynamics, physiological and trait regulatory mechanisms, as well as molecular breeding studies in P. ostreatus.

Indexed as

Chromosomes, FungalGenome, FungalGenomicsPleurotusChromosome MappingMolecular Sequence Annotationchromosome-level genomecomparative genomic analysisfunctional annotationPacBio Sequel II sequencingPleurotus ostreatuswhole-genome sequencing

Identifiers

PMID42274239

DOI10.1093/g3journal/jkag150

PMCPMC13439954

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.