Evidence map›Paper›PMID 42275220›Full record

ArticleCell reports2026

Cell size-dependent mRNA transcription drives proteome remodeling.

Dong Shin You, Christopher H Bohrer, Purva H Rumde, Ioannis Sanidas, Matthew P Swaffer, Daniel R Larson, Josh E Elias, Michael C Lanz, Jan M Skotheim

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Cell size modulates ferroptosis susceptibility.bioRxiv : the preprint server for biology · 2026
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Dong Shin YouDepartment of Biology, Stanford University, Stanford, CA 94305, USA.
Christopher H BohrerCenter for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA.
Purva H RumdeKrantz Family Center for Cancer Research, Massachusetts General Hospital, Boston, MA 02114, USA.
Ioannis SanidasKrantz Family Center for Cancer Research, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.
Matthew P SwafferDepartment of Biology, Stanford University, Stanford, CA 94305, USA; Centre for Cell Biology, Michael Swann Building, University of Edinburgh, Edinburgh EH9 3BF, UK.
Daniel R LarsonCenter for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA.
Josh E EliasChan Zuckerberg Biohub San Francisco, Stanford University, Stanford, CA 94305, USA.
Michael C LanzDepartment of Biology, Stanford University, Stanford, CA 94305, USA; Chan Zuckerberg Biohub San Francisco, Stanford University, Stanford, CA 94305, USA. Electronic address: mikelanz@stanford.edu.
Jan M SkotheimDepartment of Biology, Stanford University, Stanford, CA 94305, USA; Chan Zuckerberg Biohub San Francisco, Stanford University, Stanford, CA 94305, USA. Electronic address: skotheim@stanford.edu.

Funding

Project 3: Defining and targeting mechanisms of E2F transcription factor regulationP01CA254867 · NCI · STANFORD UNIVERSITY · PI Jan M Skotheim · 2022 to 2026
$8.9M
Determining how cell growth triggers cell divisionR35GM134858 · NIGMS · STANFORD UNIVERSITY · PI Jan M Skotheim · 2020 to 2026
$4.1M
ImageXpress Micro (IXM) Confocal High-content Imaging SystemS10OD026899 · OD · STANFORD UNIVERSITY · PI SOLOW-CORDERO, DAVID EDWARD · 2019 to 2019
$541k
NCI NIH HHS P01 CA254867NIGMS NIH HHS R35 GM134858NIH HHS S10 OD026899
6 · The paper itself

Abstract

Increasing cell size drives proteomic changes that impact cell physiology. However, the molecular basis of size-dependent proteome remodeling has remained unclear. Here, we develop an inducible Cyclin D1 expression system in human cells to generate proliferating cells spanning over a 2-fold size range. We use this system to make comprehensive genome-wide measurements of mRNA and protein concentrations and stability. We find that protein and mRNA turnover rates are weakly related to cell size but that mRNA concentrations are strongly size-dependent. This establishes that transcriptional regulation is the basis of proteome remodeling. Live-cell imaging of nascent mRNAs using the MS2 system is used to measure how transcriptional dynamics change with cell size. Larger cells prolong transcriptional bursts but maintain similar burst amplitudes to achieve transcriptional scaling. Together, our results show how transcription is modulated by cell size to remodel the proteome and alter cell physiology.

Indexed as

Cell SizeProteomeRNA, MessengerTranscription, GeneticCyclin D1HumansCyclin D1ProteomeRNA, Messengerburstingcell biologycell sizeCP: molecular biologyhomeostasislysosomescalingsingle-molecule imagingtranscriptionturnover

Identifiers

PMID42275220
PMCPMC13363025

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.