Evidence map›Paper›PMID 42278628›Full record

ArticleInternational journal of molecular sciences2026

β-Hydroxybutyrate Inhibits Angiogenesis, Suppresses Non-Small Cell Lung Cancer Growth, and Enhances Gemcitabine Antitumor Activity.

Yomna Labanie, Kholoud Arafat, Shahrazad Sulaiman, Aya Mudhafar Al-Azawi, Samir Attoub

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yomna LabanieDepartment of Pharmacology & Therapeutics, College of Medicine & Health Sciences, United Arab Emirates University, Al-Ain 15551, United Arab Emirates.
Kholoud ArafatDepartment of Pharmacology & Therapeutics, College of Medicine & Health Sciences, United Arab Emirates University, Al-Ain 15551, United Arab Emirates.
Shahrazad SulaimanDepartment of Pharmacology & Therapeutics, College of Medicine & Health Sciences, United Arab Emirates University, Al-Ain 15551, United Arab Emirates.
Aya Mudhafar Al-AzawiDepartment of Pharmacology & Therapeutics, College of Medicine & Health Sciences, United Arab Emirates University, Al-Ain 15551, United Arab Emirates.
Samir AttoubDepartment of Pharmacology & Therapeutics, College of Medicine & Health Sciences, United Arab Emirates University, Al-Ain 15551, United Arab Emirates.

Funding

College of Medicine and Health Sciences (CMHS) / United Arab Emirates University, No. 12M177. This research was partially supported by ASPIRE, the technology program management pillar of Abu Dhabi's Advanced Technology Research Council (ATRC), via the ASP No. 12M177 and VRI-20-10
6 · The paper itself

Abstract

Lung cancer remains the most prevalent malignancy and the leading cause of cancer-related mortality worldwide, highlighting the need for novel therapeutic strategies. β-Hydroxybutyrate (BHB), the primary circulating ketone body, has shown antitumor activity in other cancers, but its role in non-small cell lung cancer (NSCLC) is not well defined. This study investigated the anticancer and anti-angiogenic effects of BHB alone and in combination with Gemcitabine using in vitro and in vivo approaches. In A549 (adenocarcinoma) and LNM35 (large-cell carcinoma) NSCLC cells, BHB significantly reduced cell number and colony growth in a concentration-dependent manner, with LNM35 cells exhibiting greater sensitivity. Migration of both A549 and LNM35 cells was also markedly inhibited. In endothelial Telo-HAEC cells, BHB exhibited no cytotoxicity but significantly inhibited migration, tube formation, and VEGF-induced spheroid sprouting, indicating anti-angiogenic activity. Combination studies showed that BHB enhanced Gemcitabine-mediated suppression of NSCLC cell number and colony growth, consistent with an additive to synergistic effect. In the chick embryo chorioallantoic membrane model, BHB and Gemcitabine reduced tumor growth individually, while their combination further decreased tumor weight, particularly in LNM35 xenografts, without observable toxicity. These findings provide preclinical evidence that BHB enhances Gemcitabine antitumor activity against NSCLC and reveals its in vitro anti-angiogenic effects.

Indexed as

3-Hydroxybutyric AcidAngiogenesis InhibitorsCarcinoma, Non-Small-Cell LungDeoxycytidineLung NeoplasmsNeovascularization, PathologicA549 CellsAnimalsCell Line, TumorCell MovementCell ProliferationChick EmbryoDrug SynergismGemcitabineHumansMice3-Hydroxybutyric AcidAngiogenesis InhibitorsDeoxycytidineGemcitabineangiogenesiscolony growthgemcitabinemigrationNSCLCtumor growthβ-hydroxybutyrate

Identifiers

PMID42278628
PMCPMC13258558

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.