Evidence map›Paper›PMID 42282825›Full record

ArticlebioRxiv : the preprint server for biology2026

Combinatorial and Inducible CRISPRa/i Enables Canalized hiPSC Forward Programming and Iterative Refinement

Federica Sozza, Alberto Romano, Nicole D'Elia, Martina Terenzi, Maria Luisa Ratto, Emily R Cliff, Gabrielle Nattenberg, Sara Bianchi, Silvia Becca, Heather Klug and 4 more

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Federica SozzaMolecular Biotechnology Center "Guido Tarone", Department of Molecular Biotechnology and Health Sciences, University of Turin, Torino, Italy.ORCID 0009-0008-2405-769X
Alberto RomanoMolecular Biotechnology Center "Guido Tarone", Department of Molecular Biotechnology and Health Sciences, University of Turin, Torino, Italy.ORCID 0009-0009-8800-4164
Nicole D'EliaMolecular Biotechnology Center "Guido Tarone", Department of Molecular Biotechnology and Health Sciences, University of Turin, Torino, Italy.
Martina TerenziMolecular Biotechnology Center "Guido Tarone", Department of Molecular Biotechnology and Health Sciences, University of Turin, Torino, Italy.
Maria Luisa RattoMolecular Biotechnology Center "Guido Tarone", Department of Molecular Biotechnology and Health Sciences, University of Turin, Torino, Italy.ORCID 0000-0003-2610-242X
Emily R CliffDepartment of Chemistry, University of Washington, Seattle, WA, USA.ORCID 0009-0009-4796-4177
Gabrielle NattenbergDepartment of Laboratory Medicine & Pathology, University of Washington, Seattle WA USA.
Sara BianchiMolecular Biotechnology Center "Guido Tarone", Department of Molecular Biotechnology and Health Sciences, University of Turin, Torino, Italy.ORCID 0009-0002-8947-0342
Silvia BeccaMolecular Biotechnology Center "Guido Tarone", Department of Molecular Biotechnology and Health Sciences, University of Turin, Torino, Italy.ORCID 0009-0009-4918-1133
Heather KlugDepartment of Laboratory Medicine & Pathology, University of Washington, Seattle WA USA.
Davide CacchiarelliTelethon Institute of Genetics and Medicine (TIGEM), Pozzuoli; Department of Translational Medicine, University of Naples Federico II and Scuola Superiore Meridionale, Naples, Italy.ORCID 0000-0002-9621-6716
Jesse G ZalatanDepartment of Chemistry, University of Washington, Seattle, WA, USA.ORCID 0000-0002-1458-0654
Elisa BalmasMolecular Biotechnology Center "Guido Tarone", Department of Molecular Biotechnology and Health Sciences, University of Turin, Torino, Italy.ORCID 0000-0002-4600-6809
Alessandro BerteroMolecular Biotechnology Center "Guido Tarone", Department of Molecular Biotechnology and Health Sciences, University of Turin, Torino, Italy.ORCID 0000-0002-4919-9087

Funding

University of Washington Center for Nuclear Organization and FunctionU54DK107979 · NIDDK · UNIVERSITY OF WASHINGTON · PI NOBLE, WILLIAM STAFFORD, SHENDURE, JAY ASHOK · 2015 to 2019
$11.8M
Spatial Organization in Biochemical Reaction NetworksR35GM124773 · NIGMS · UNIVERSITY OF WASHINGTON · PI Jesse George Zalatan · 2017 to 2026
$3.9M
NIDDK NIH HHS U54 DK107979NIGMS NIH HHS R35 GM124773
6 · The paper itself

Abstract

Synthetic gene-regulation logic is established in immortalized cell lines but remains largely aspirational in human induced pluripotent stem cells (hiPSCs) and derivatives. This gap constrains both mechanistic discovery and translational engineering in physiologically relevant models. We developed

Identifiers

PMID42282825
PMCPMC13252116

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.