Evidence map›Paper›PMID 42284134›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2026

Wound Healing and Angiogenic Profiling of Dermal Endothelial Cells Isolated From People With Type 2 Diabetes.

James Shadiow, Corey E Mazo, Pallavi Varshney, Jeongjin J Kim, Alexander Ahn, Sophie H Chong, Cory A Lutz, Crystal M Holmes, Michael E Munson, Brian M Schmidt and 5 more

Abstract read
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In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

James ShadiowSchool of Kinesiology, University of Michigan, Ann Arbor, Michigan, USA.
Corey E MazoSchool of Kinesiology, University of Michigan, Ann Arbor, Michigan, USA.
Pallavi VarshneySchool of Kinesiology, University of Michigan, Ann Arbor, Michigan, USA.
Jeongjin J KimSchool of Kinesiology, University of Michigan, Ann Arbor, Michigan, USA.
Alexander AhnSchool of Kinesiology, University of Michigan, Ann Arbor, Michigan, USA.ORCID https://orcid.org/0009-0009-7865-2061
Sophie H ChongSchool of Kinesiology, University of Michigan, Ann Arbor, Michigan, USA.
Cory A LutzSchool of Kinesiology, University of Michigan, Ann Arbor, Michigan, USA.
Crystal M HolmesDivision of Metabolism, Endocrinology, and Diabetes, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Michael E MunsonDivision of Metabolism, Endocrinology, and Diabetes, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Brian M SchmidtDivision of Metabolism, Endocrinology, and Diabetes, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Sascha N GoonewardenaAnn Arbor Veterans Affairs Health System, Ann Arbor, Michigan, USA.
Richard D MinshallDepartments of Anesthesiology, Pharmacology and Regenerative Medicine, University of Illinois at Chicago College of Medicine, Chicago, Illinois, USA.
Thomas P J SolomonBlazon ScientificWimbledon, London, UK.
Andrew T LudlowSchool of Kinesiology, University of Michigan, Ann Arbor, Michigan, USA.ORCID https://orcid.org/0000-0003-0423-4800
Jacob M HausSchool of Kinesiology, University of Michigan, Ann Arbor, Michigan, USA.ORCID https://orcid.org/0000-0002-8048-2470

Funding

HHS | National Institutes of Health (NIH) DK020572HHS | National Institutes of Health (NIH) DK109948
6 · The paper itself

Abstract

Impaired wound healing in type 2 diabetes (T2D) is associated with microvascular dysfunction and remains a significant clinical challenge. We aimed to determine whether primary human dermal microvascular endothelial cells (HDMVECs) from individuals with T2D exhibit abnormal cellular functions, and whether exposure to T2D serum impacts healthy endothelial function. In Experiment 1, T2D-HDMVECs displayed paradoxically higher migratory and angiogenic capacities than their healthy counterparts, despite markedly reduced eNOS expression and disrupted endothelial-identity gene expression. In Experiments 2 and 3, healthy HDMVECs showed decreased tube formation, nitric oxide production, and Notch/angiogenesis-related gene expression after exposure to both healthy and T2D serum, suggesting the presence of serum-derived factors that suppress these pathways. However, T2D-HDMVECs remained largely unresponsive to these serum-driven effects, reinforcing an intrinsic reprogramming of T2D endothelial cells. Additional analyses revealed selective alterations in redox and angiogenic signaling pathways (e.g., NOX4, FLT1), whereas canonical regulators such as VEGFA and PFKB3 were not affected by serum exposure. Overall, our data reveal a complex interplay between cell-autonomous alterations and extrinsic signals in diabetic endothelial dysfunction. Therapeutic strategies targeting both intrinsic cellular programs (e.g., eNOS, Notch signaling) and the circulating milieu may represent promising avenues for enhancing wound repair in patients with T2D.

Indexed as

AngiogenesisDermisDiabetes Mellitus, Type 2Endothelial CellsSkinWound HealingCell MovementCells, CulturedFemaleHumansMaleMiddle AgedNitric OxideNitric Oxide Synthase Type IIISignal TransductionNitric OxideNitric Oxide Synthase Type IIIangiogenesisdermal microvascular endothelial cellsdiabetic foot ulcersendothelial dysfunctionnotch signalingtype 2 diabeteswound healing

Identifiers

PMID42284134
PMCPMC13262748

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.