Evidence mapPaperPMID 42288013Full record

ArticleInternational dental journal2026

Mitochondrial Metabolic Biomarkers in Periodontitis: Discovery and Clinical Validation.

Jiahua Wu, Juan Wu, Yubing Hong, Lijia Rao, Mu Chen

Abstract readValidation Study
In one paragraph

Article in International dental journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Jiahua WuDepartment of Stomatology, Shenzhen Nanshan People's Hospital, Shenzhen, Guangdong, 518000, China.
Juan WuDepartment of Stomatology, Shenzhen Nanshan People's Hospital, Shenzhen, Guangdong, 518000, China.
Yubing HongDepartment of Stomatology, Shenzhen Nanshan People's Hospital, Shenzhen, Guangdong, 518000, China.
Lijia RaoDepartment of Stomatology, Shenzhen Nanshan People's Hospital, Shenzhen, Guangdong, 518000, China.
Mu ChenDepartment of Stomatology, Shenzhen Nanshan People's Hospital, Shenzhen, Guangdong, 518000, China. Electronic address: drchenmu@foxmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

INTRODUCTION AND

aimsMitochondrial metabolic dysregulation is associated with periodontitis (PD); however, related biomarkers remain unclear. In this study, we identified key mitochondrial metabolism-related biomarkers in PD through integrated bioinformatics technology.

methodsTranscriptomic and single-cell RNA sequencing data from the Gene Expression Omnibus database were analysed using machine learning to identify biomarkers. A diagnostic nomogram was constructed. Immune infiltration, drug prediction, and T-cell dynamic expression were assessed. Putative biomarkers were validated in clinical samples and a rat PD model by reverse transcription-quantitative polymerase chain reaction, immunohistochemistry, and Western blot analysis.

resultsSix biomarkers were identified, which included ENTPD1, CYP24A1, ADA, TDO2 (upregulated), OSBPL6, and NUDT15 (downregulated). The nomogram exhibited excellent diagnostic efficacy (area under the curve [AUC] = 0.954). The biomarkers correlated with plasma cells and resting dendritic cells. Single-cell analysis revealed dynamic expression during T-cell differentiation. CTA018 exhibited strong binding to CYP24A1. Five biomarkers were validated in clinical samples, and the upregulation of ENTPD1, CYP24A1, and TDO2 protein and messenger RNA was confirmed in a rat model and correlated with PD progression.

conclusionENTPD1, CYP24A1, and TDO2 are mitochondrial metabolism-related biomarkers with high diagnostic potential in PD and targets for therapy. CLINICAL RELEVANCE: The identified biomarkers, particularly ENTPD1, CYP24A1, and TDO2, have strong diagnostic value. The nomogram contributed to PD risk stratification. Associations with immune infiltration and drug sensitivity may guide immunotherapy and targeted therapy.

Indexed as

BiomarkersMitochondriaPeriodontitisAnimalsBlotting, WesternComputational BiologyDisease Models, AnimalHumansImmunohistochemistryMaleNomogramsRatsRats, Sprague-DawleyBiomarkersAnimal modelBiomarkersMachine learningMitochondrial metabolismPeriodontitisSingle-cell RNA sequencing

Identifiers

PMID42288013
PMCPMC13279168

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.