Single-granule profiling reveals that RNP granule pH marks cellular translation.

Yue Zhang, Shining Wu, Yifeng He, Xuhan Xia, Jinchao Wei, Shijia Wu, Ruijie Deng

Abstract read

In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yue ZhangCollege of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China.

Shining WuCollege of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China.

Yifeng HeCollege of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China.

Xuhan XiaCollege of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China.

Jinchao WeiMacau Centre for Research and Development in Chinese Medicine, State Key Laboratory of Mechanism and Quality of Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China.

Shijia WuState Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Ruijie DengCollege of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ribonucleoprotein (RNP) granules reshape RNA metabolism in eukaryotic cells, yet their biophysical properties remain poorly understood due to the lack of probes targeting these small, dynamic condensates. Herein, we developed RNP granule-pH (RNPg-pH), a ratiometric and targetable probe, to map the pH of processing bodies (P-bodies) and stress granules (SGs) at the single-granule level. This approach revealed a spatial pH heterogeneity within RNP granules, with a more alkaline periphery and a more acidic region near the nucleus. Stresses including oxidative stress, hyperosmotic stress, and translational inhibition induced granule acidification and reduced translation level, suggesting that RNPg-pH served as a sensitive marker of translation activity. Notably, compounds like myrtenol and oridonin reversed hydrogen peroxide (H

Indexed as

cell biologymolecular biology

Identifiers

PMID42291224

DOI10.1016/j.isci.2026.116203

PMCPMC13254902

What Socratic holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.