Evidence map›Paper›PMID 42298623›Full record

ArticleBiomedical engineering online2026

Potentials of BMSCs for regulating osteogenic-vascular-neural-lymphatic coupling in bone regeneration.

Nan Zhang, Yuwen Luo, Peng Luo, Jiarui Cao, Yuning Cheng, Mengyao Kang, Jianping Mao, Jing-Jun Nie, Da-Fu Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical engineering online, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Nan Zhang *Laboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.ORCID http://orcid.org/0009-0004-4753-1375
Yuwen Luo *Laboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.ORCID http://orcid.org/0009-0007-5108-6392
Peng LuoLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.ORCID http://orcid.org/0009-0009-6905-4529
Jiarui CaoLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.ORCID http://orcid.org/0009-0000-6093-7709
Yuning ChengLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.ORCID http://orcid.org/0000-0002-8171-8748
Mengyao KangLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.ORCID http://orcid.org/0009-0000-4435-0027
Jianping MaoDepartment of Spine Surgery, National Center for Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.
Jing-Jun NieLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China. niejingjun_jst@126.com.ORCID http://orcid.org/0000-0002-1653-0976
Da-Fu ChenLaboratory of Bone Tissue Engineering, Beijing Laboratory of Biomedical Materials, National Center for Orthopaedics, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China. chendafujst@126.com.ORCID http://orcid.org/0000-0002-1879-8239

Funding

Beijing Jishuitan Hospital Program JSTYC202206Beijing Jishuitan Hospital Program ZR-202305Beijing Natural Science Foundation Z250013National Key Research and Development Program of China 2022YFA1207500National Natural Science Foundation of China 52273121National Natural Science Foundation of China 52373128the Beijing Municipal Public Welfare Development and Reform Pilot Project for Medical Research Institutes JYY2023-11
6 · The paper itself

Abstract

backgroundBone regeneration assisted by synthetic bone substitutes largely depends on the integration of the vascular, neural, and lymphatic systems in the bone. Bone marrow mesenchymal stem cells (BMSCs) are the key cells for this process. However, their role in regulating the integration has not been fully characterized.

methodsHuman BMSCs (hBMSCs) were treated with osteogenic induction and collected from 0 to 504 h for bulk RNA sequencing (RNA-Seq). Differentially expressed genes (DEGs) were identified and Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Time-Series Transcriptomic Trend Analysis were used to comprehensively analyze the possible pathways and functions associated with these DEGs. Weighted Gene Co-expression Network Analysis (WGCNA) was constructed to identify the modules and hub genes of the process. Quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) were performed to validate the expression of key genes identified by RNA-Seq.

resultsTime-series analysis of the hBMSCs transcriptome suggested a dynamic expression trajectory during osteogenic differentiation, which was characterized by four functional patterns: the initial adaptation stage (1-24 h), the proliferation activation stage (24-72 h), the differentiation regulation stage (72-336 h) and the remodeling stability stage (336-504 h). Moreover, 72 h was suggested as a potential key time point in the osteogenic-vascular-neural-lymphatic coupling process based on transcriptomic analysis, with typical activation of BMP, vascular endothelial growth factor (VEGF) and PPAR signaling pathways. Four modules and closely related hub genes such as growth differentiation factor 5 (GDF5), matrix Gla protein (MGP) and pregnancy-associated plasma protein A2 (PAPPA2), whose expressions were validated by qRT-PCR and ELISA were also identified and highlighted.

conclusionsOur study revealed the temporal trends of angiogenesis, lymphangiogenesis, and neurogenesis during BMSCs osteogenic differentiation, which not only supplemented the transcriptional regulation in bone regeneration, but also provided a theoretical basis for the design of synthetic bone substitutes.

Indexed as

Bone RegenerationMesenchymal Stem CellsOsteogenesisCell DifferentiationGene Expression ProfilingGene Expression RegulationHumansBone regenerationCell communicationMesenchymal stem cellsOsteogenesisRNA-Seq

Identifiers

PMID42298623
PMCPMC13501691

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.