Imaging-Guided Live Single-Cell Lipid Profiling of Leader and Follower Cells During Collective Migration of Triple-Negative Breast Cancer Cells.

Xiaoyue Huang, Judit González Riera, Mai Nguyen, Jeremy Broekhuis, Sylvia E Le Dévédec, Ahmed Ali, Thomas Hankemeier

Abstract read

In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xiaoyue HuangMetabolomics and Analytics Centre, Leiden Academic Centre For Drug Research, Leiden University, Leiden, The Netherlands.

Judit González RieraMetabolomics and Analytics Centre, Leiden Academic Centre For Drug Research, Leiden University, Leiden, The Netherlands.

Mai NguyenMetabolomics and Analytics Centre, Leiden Academic Centre For Drug Research, Leiden University, Leiden, The Netherlands.

Jeremy BroekhuisInstitute of Biology, Leiden University, Leiden, The Netherlands.

Sylvia E Le DévédecDivision of Cell Systems and Drug Safety, Leiden Academic Centre For Drug Research, Leiden University, Leiden, The Netherlands.ORCID https://orcid.org/0000-0002-0615-9616

Ahmed AliMetabolomics and Analytics Centre, Leiden Academic Centre For Drug Research, Leiden University, Leiden, The Netherlands.ORCID https://orcid.org/0000-0003-2157-4399

Thomas HankemeierMetabolomics and Analytics Centre, Leiden Academic Centre For Drug Research, Leiden University, Leiden, The Netherlands.

Funding

China Scholarship Council 202206280025NWO XS OCENW.XS23.1.070

6 · The paper itself

Abstract

Leader and follower cells drive collective cancer cell migration through distinct and interactive roles, but their specific metabolic signatures remain largely unknown. Here, we developed and applied a workflow that integrates time-lapse imaging, single-cell tracking, microsampling and direct infusion high-resolution mass spectrometry to profile the lipidomes of individually sampled leader and follower cells from highly migratory TNBC lines HCC1143 and HCC38. Approximately 120 metabolites were detected per cell at the MS

Indexed as

collective migrationdirect infusionlipid profilinglive single‐cellnano‐ESITNBC

Identifiers

PMID42301727

DOI10.1002/advs.75862

PMCPMC13336123

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.