Evidence map›Paper›PMID 42301820›Full record

ArticleSTAR protocols2026

Protocol to quantify desmoglein 3 tension at cell-cell adhesions using FRET-based tension sensors.

Bahareh Tajvidi Safa, Timothy Goldsmith, Zachary Kezhaya, Sarah Seman, Daniel E Conway, Ruiguo Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Bahareh Tajvidi SafaDepartment of Biomedical Engineering, Michigan State University, East Lansing, MI 48823, USA; Institute for Quantitative Health Science and Engineering (IQ), Michigan State University, East Lansing, MI 48823, USA.
Timothy GoldsmithDepartment of Biomedical Engineering, Michigan State University, East Lansing, MI 48823, USA; Institute for Quantitative Health Science and Engineering (IQ), Michigan State University, East Lansing, MI 48823, USA.
Zachary KezhayaDepartment of Biomedical Engineering, Michigan State University, East Lansing, MI 48823, USA.
Sarah SemanDepartment of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA.
Daniel E ConwayDepartment of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA; The Ohio State University and Arthur G. James Comprehensive Cancer Center, Columbus, OH 43210, USA. Electronic address: conway.362@osu.edu.
Ruiguo YangDepartment of Biomedical Engineering, Michigan State University, East Lansing, MI 48823, USA; Institute for Quantitative Health Science and Engineering (IQ), Michigan State University, East Lansing, MI 48823, USA. Electronic address: yangruig@msu.edu.

Funding

Cell junction and nuclear forces as mediators of epithelial cell homeostasisR35GM119617 · NIGMS · VIRGINIA COMMONWEALTH UNIVERSITY · PI Daniel E Conway · 2016 to 2026
$4.5M
Measurement of Mechanical Tension Across DesmosomesR03AR068096 · NIAMS · VIRGINIA COMMONWEALTH UNIVERSITY · PI CONWAY, DANIEL E · 2016 to 2018
$212k
NIAMS NIH HHS R03 AR068096NIGMS NIH HHS R35 GM119617
6 · The paper itself

Abstract

Desmoglein 3 (Dsg3) is a crucial component of cell-cell junctions, contributing to mechanotransduction and maintaining tissue integrity. Here, we present a protocol to quantify tension at keratinocyte cell-cell adhesion sites using a Dsg3 fluorescence resonance energy transfer (FRET)-based tension sensor. We describe steps for increasing viral titer by amplifying adenovirus in HEK293A cells and optimizing infection conditions to ensure uniform expression of FRET signals. We detail procedures for live-cell imaging of keratinocytes and image analysis to quantify the FRET ratio. For complete details on the use and execution of this protocol, please refer to Jin et al.

Indexed as

BiophysicsBiotechnology and bioengineeringCell Biology

Identifiers

PMID42301820
PMCPMC13292546

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.