Evidence mapPaperPMID 42318532Full record

ArticleKidney international reports2026

IgM Hyposialylation Modulates Podocyte Vulnerability in Patients With Idiopathic Nephrotic Syndrome.

Sonia Spinelli, Sofia Gaudiano, Andrea Garbarino, Francesca Lugani, Edoardo La Porta, Andrea Petretto, Martina Bartolucci, Chiara Lavarello, Nicole Grinovero, Ilaria Musante and 10 more

Abstract read
In one paragraph

Article in Kidney international reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Sonia SpinelliUnit of Nephrology, Dialysis, and Transplantation, Laboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, Italy.
Sofia GaudianoDepartment of Experimental Medicine (DIMES), University of Genoa, Italy.
Andrea GarbarinoUnit of Nephrology, Dialysis, and Transplantation, Laboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, Italy.
Francesca LuganiUnit of Nephrology, Dialysis, and Transplantation, Laboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, Italy.
Edoardo La PortaUnit of Nephrology, Dialysis, and Transplantation, Laboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, Italy.
Andrea PetrettoCore Facility for Omics Sciences, IRCCS Istituto Giannina, Genoa, Italy.
Martina BartolucciCore Facility for Omics Sciences, IRCCS Istituto Giannina, Genoa, Italy.
Chiara LavarelloCore Facility for Omics Sciences, IRCCS Istituto Giannina, Genoa, Italy.
Nicole GrinoveroCore Facility for Omics Sciences, IRCCS Istituto Giannina, Genoa, Italy.
Ilaria MusanteMedical Genetics Unit, IRCCS Istituto Giannina Gaslini, Genoa, Italy.
Paolo ScudieriMedical Genetics Unit, IRCCS Istituto Giannina Gaslini, Genoa, Italy.
Antonella TrivelliUnit of Nephrology, Dialysis, and Transplantation, Laboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, Italy.
Giorgio PiaggioUnit of Nephrology, Dialysis, and Transplantation, Laboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, Italy.
Alberto MagnascoUnit of Nephrology, Dialysis, and Transplantation, Laboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, Italy.
Maria Ludovica Degl'InnocentiUnit of Nephrology, Dialysis, and Transplantation, Laboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, Italy.
Simona GranataDepartment of Biology, Ecology and Earth Sciences, University of Calabria, Rende, Italy.
Gianluigi ZazaDivision of Nephrology, Department of Translational and Precision Medicine, Policlinico Umberto I Hospital, Sapienza University of Rome, Rome, Italy.
Enrico VerrinaUnit of Nephrology, Dialysis, and Transplantation, Laboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, Italy.
Giovanni CandianoUnit of Nephrology, Dialysis, and Transplantation, Laboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, Italy.
Maurizio BruschiUnit of Nephrology, Dialysis, and Transplantation, Laboratory of Molecular Nephrology, IRCCS Istituto Giannina Gaslini, Genoa, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Altered Ig glycosylation has been implicated in antibody-mediated podocytopathies; however, the functional relevance of IgM sialylation remains poorly defined. Previous evidence suggests that circulating cationic or hyposialylated IgM may contribute to podocyte vulnerability in idiopathic nephrotic syndrome (iNS). Methods: Serum IgM from 86 pediatric and adult patients with podocytopathies, 20 patients with membranous nephropathy (MN), 20 patients with lupus nephritis (LN), and 30 healthy controls were analyzed by lectin-based enzyme-linked immunosorbent assay (ELISA) using biotinylated lectins to assess terminal N-glycan residues, including Sambucus nigra agglutinin (SNA) and Ricinus communis agglutinin I (RCA-I). Serum levels of the sialyltransferase ST6GAL1 and the sialidases neuraminidase-1 (NEU1) and neuraminidase-3 (NEU3) were quantified. Cultured human podocytes were exposed to native, desialylated, or resialylated IgM and analyzed by confocal microscopy, quantitative proteomics, phosphoproteomics, and metabolic assays. Results: IgM from patients with iNS showed reduced SNA binding, which inversely correlated with proteinuria and circulating NEU1/NEU3 levels. In paired samples, SNA reactivity decreased during relapse and increased during remission. ST6GAL1 was undetectable across all groups, whereas phospholipase A2 receptor 1(PLA2R1)-positive MN displayed reduced RCA-I binding. Podocytes exposed to hyposialylated or desialylated IgM exhibited disorganization of the actin cytoskeleton, reduced nephrin signal, increased lipid peroxidation, and decreased ATP levels. Resialylated IgM displayed podocyte morphological and metabolic features not statistically distinguishable from those observed under control conditions. Proteomic and phosphoproteomic analyses highlighted modulation of mitogen-activated protein kinase (MAPK)-, mechanistic Target of Rapamycin (mTOR-), adenosine monophosphate-activated protein kinase (AMPK), and cytoskeleton-related pathways. Conclusion: IgM sialylation status tracks disease activity and modulates podocyte structural, metabolic, and signaling responses, supporting immune glycan remodeling as a disease-associated modifier of podocyte vulnerability in iNS.

Indexed as

hyposialylationIgM glycosylationpodocyte injurypodocytopathiessialidase

Identifiers

PMID42318532
PMCPMC13273652

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.