ArticleNature communications2026
Single-molecule imaging reveals RNA polymerase II dynamics and TAF1-dependent promoter-proximal pause release.
Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Single-molecule imaging reveals RNA polymerase II dynamics and TAF1-dependent promoter-proximal pause release.Nature communications · 2026Article
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Authors and funding
4 authors.
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Abstract
Early transcriptional regulation is a major kinetic bottleneck in gene expression, yet the dynamics of RNA polymerase II in living cells remain poorly understood. Here, we use single-molecule imaging to quantify RNA polymerase II chromatin engagement in live human cells. We find that RNA polymerase II infrequently enters a long-lived binding state associated with productive elongation, with ~94% of chromatin-associated events dissociating within tens of seconds. Single-cell analysis reveals substantial heterogeneity in the fraction of RNA polymerase II reaching productive elongation. Spatial heatmap analysis identifies RNA polymerase II clusters enriched for repeated long-lived binding events whose abundance is selectively perturbed by transcriptional inhibitors. Acute degradation of the TFIID subunit TAF1 reduces RNA polymerase II clustering and increases the global pausing index, supporting a model in which TAF1 promotes pause release and entry into productive elongation. Together, these findings provide a single-molecule framework for quantifying transcription kinetics in individual living cells.
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Registered trials
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