ArticleAmerican journal of translational research2026
GM-CSF and IL-1α secreted by cryopreserved porcine skin promote angiogenesis in burn wounds by activating the JAK2/STAT3 pathway.
Article in American journal of translational research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveCryopreserved porcine skin (CPS) is widely applied in skin grafting for burns. In this study, we aimed to investigate the effects and underlying mechanisms of CPS on burn wound healing.
methodsCryopreserved porcine skins were acquired. Guinea pig burn models were established and grafted with thawed-CPS. Human umbilical vein endothelial cells (HUVECs) were cultured in CPS-conditioned medium. Histological structure was assessed by hematoxylin-eosin (HE) staining. Protein levels were detected using enzyme-linked immunosorbent assay (ELISA), western blot, and immunohistochemistry. HUVECs proliferation was evaluated by 5-ethynyl-2'-deoxyuridine (EdU) assay. The invasion and migration of HUVECs were analyzed via Transwell assay. Tube formation was also assessed.
resultsCPS maintained its structural integrity and cellular viability, with lower antigenicity compared with fresh porcine skin. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-1 alpha (IL-1α) secreted by CPS were detected in the CPS-grafted burned tissue. Moreover, CPS grafting accelerated wound healing and angiogenesis in burned guinea pigs. In vitro, CPS-conditioned medium enhanced the proliferation, invasion, migration, and tube formation of HUVECs, as well as activated the JAK2/STAT3 pathway. Neutralizing antibodies against GM-CSF and IL-1α synergistically reversed the pro-angiogenic effects of CPS in vivo and in vitro. Additionally, JAK2 inhibitor AG490 abolished the enhanced proliferation, invasion, migration, and tube formation of HUVECs induced by CPS-conditioned medium.
conclusionWe confirmed that CPS-secreted GM-CSF and IL-1α promoted angiogenesis in burn tissue by activating the JAK2/STAT3 pathway, thereby revealing an active therapeutic role of CPS for burn wound healing and offering theoretical support for its clinical application.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.