ArticlebioRxiv : the preprint server for biology2026
Robust mammalian RNA localization elements are complex and multipartite.
Charlie Moffatt, Sharifah Syed Salim, Karl Bauer, Andrea MacFadden, Alli Jimenez, Chase Weidmann, Allison W McClure, Daniel Dominguez, Michael Kiebler, J Matthew Taliaferro
Abstract readPreprint
In one paragraphArticle in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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0citing papers in PubMed
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What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
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3 · Its place in the literatureWho cites it
0 citing papers in PubMed.
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4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
10 authors.
Charlie MoffattDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Sharifah Syed SalimBiomedical Center (BMC), Department for Cell Biology, Medical Faculty, Ludwig-Maximilians-University of Munich, Germany.
Karl BauerBiomedical Center (BMC), Department for Cell Biology, Medical Faculty, Ludwig-Maximilians-University of Munich, Germany.
Andrea MacFaddenDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Alli JimenezDepartment of Pharmacology, University of North Carolina at Chapel Hill, USA.
Chase WeidmannDepartment of Biological Chemistry, Center for RNA Biomedicine, University of Michigan Medical School, USA.ORCID 0000-0002-2941-0500 Allison W McClureDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Daniel DominguezDepartment of Pharmacology, University of North Carolina at Chapel Hill, USA.
Michael KieblerBiomedical Center (BMC), Department for Cell Biology, Medical Faculty, Ludwig-Maximilians-University of Munich, Germany.
J Matthew TaliaferroDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.ORCID 0000-0001-7580-1433 Funding
Predoctoral Training Program in Molecular and Cellular Biology (Supplement: Mentoring in the Research Environment)T32GM136444 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI MICHAEL A MCMURRAY, Rytis Prekeris · 2020 to 2026
$3.7MUnderstanding the regulatory language of RNA localizationR35GM133385 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI Jefferson Matthew Taliaferro · 2019 to 2026
$3.4MProtein Disorder as a Modifier of RNA Binding and RegulationR35GM142864 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Daniel Issac Dominguez · 2021 to 2026
$2.5MMechanistic analysis of TDP-43-mediated RNA localization in neurons and its misregulation in ALSR01NS122911 · NINDS · UNIVERSITY OF COLORADO DENVER · PI Holger A. Russ, Jefferson Matthew Taliaferro · 2023 to 2026
$2.3MDeveloping RNP-MaP into a broad-spectrum toolset for discovery, definition, and drug targeting of RNA-protein complexes in live cellsR01GM155542 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Chase A Weidmann · 2024 to 2026
$940kMolecular mechanisms of DNA replication in mitosisR35GM156773 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI Allison McClure · 2025 to 2026
$780kHigh-throughput dissection of RNA localization regulatory elementsF31GM151819 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI MOFFATT, CHARLIE E · 2023 to 2024
$74kNIGMS NIH HHS F31 GM151819NIGMS NIH HHS R01 GM155542NIGMS NIH HHS R35 GM133385NIGMS NIH HHS R35 GM142864NIGMS NIH HHS R35 GM156773NIGMS NIH HHS T32 GM136444NINDS NIH HHS R01 NS122911
6 · The paper itselfAbstract
The subcellular localization patterns of RNAs are controlled by regulatory elements contained within them. However, for most localized RNAs, the identities of these elements remain unknown. We had previously identified several localization elements that are necessary and sufficient for robust, kinesin-dependent RNA targeting to microtubule plus ends in a variety of cell types. Yet the characteristics of these elements that are critical for function remained unclear. To address this, we systematically created tens of thousands of mutant localization elements and quantified their ability to regulate subcellular RNA localization in neuronal cells. We found that the minimally active size of these localization elements is large, approximately 200 nucleotides. These elements contain multiple important subsequences, with some being completely intolerant of any changes and others being tolerant to a shuffling of nucleotide order but not to changes in nucleotide composition. Using single molecule microscopy, we verified these findings in primary rat neurons. Together, these results demonstrate that highly active mammalian RNA localization elements are large, complex, and multipartite and lay a foundation for further mechanistic studies of their function.
Identifiers
PMID42327238
PMCPMC13277945
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