Evidence map›Paper›PMID 42343196›Full record

ArticleBMC plant biology2026

Role and mechanisms of moisture regulation in enhancing alfalfa silage quality.

Guolin Yang, Heng Jiang, Haoran Wang, Zhennan He, Zhaoming Wang, Siyi Wang, Yuanyuan Jing, Fengqin Gao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Guolin Yang *Institute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot, 010010, China.
Heng Jiang *Tianjin University, Tianjin, 300000, China.
Haoran WangAgriculture and Engineering Department, Shanxi Yuncheng Vocational and Technical College of Agricultural, Yuncheng, 044000, China.
Zhennan HeInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot, 010010, China.
Zhaoming WangInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot, 010010, China.
Siyi WangInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot, 010010, China.
Yuanyuan JingInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot, 010010, China. jingyuanyuan@caas.cn.
Fengqin GaoInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot, 010010, China. gaofq1211@126.com.

Funding

2023 National Center of Pratacultural Technology Innovation (under preparation) Major Innovation Platform Construction Project: "Research and demonstration of key technologies for high-quality forage production and grass product processing CCPTZX2023B07Central Public Welfare Scientific Research Institutes Basic Research Fund: "Study on the Mechanism of Moisture Regulation on Protein Degradation in Alfalfa Silage" Grant No. 16103320260Supported by the earmarked fund for Inner Mongolia Agriculture and Animal Husbandry Research System IMAHRS-9The Inner Mongolia Autonomous Region Science and Technology Plan Project "Research and Applicationof Key Technologies for Efficient Utilization of Alfalfa and Straw Resources 2021GG0391
6 · The paper itself

Abstract

backgroundManaging moisture content is crucial for optimizing the fermentation quality of alfalfa silage; however, the underlying mechanisms of water spraying following wilting remain poorly understood. This study aimed to elucidate the microbial mechanisms through which this rehydration process enhances fermentation quality by modulating key microorganisms and metabolic pathways.

resultsIn this study, alfalfa was subjected to four moisture treatments: ND50 (wilted to 50% moisture content), ND65 (wilted to 65% moisture content), PS50 (wilted and water-sprayed to 50% moisture content), and PS65 (wilted and water-sprayed to 65% moisture content). Following 90 days of ensiling, changes in nutritional quality, fermentation characteristics, microbial communities, and metabolites were analyzed. Results showed that the PS65 treatment exhibited significantly lower dry matter (DM), water-soluble carbohydrates (WSC), pH, ammonia nitrogen content (NH

conclusionWithin the appropriate moisture range for alfalfa silage, the PS65 treatment not only significantly altered the relative abundances of key bacteria, including Weissella and Exiguobacterium, and fungi, including Coprinellus and Cladosporium(P < 0.05), but also significantly decreased silage pH (P < 0.05) and increased Lactic acid (LA)content (P < 0.05) by modulating metabolic pathways such as phenylpropanoid biosynthesis.

Indexed as

Medicago sativaSilageWaterAmino AcidsFermentationNutritive ValueAmino AcidsWaterAlfalfaFermentation qualityFree waterMetabolomicsMicrobial diversitySilage

Identifiers

PMID42343196
PMCPMC13295738

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.