Evidence mapPaperPMID 42353161Full record

ArticleInternational journal of molecular sciences2026

Transcriptomic Profile of Genes Regulating Cellular Response to Extra- and Intracellular Stimuli in Porcine Ovarian Granulosa Cells During In Vitro Cultivation.

Krzysztof Data, Wiesława Kranc, Małgorzata Blatkiewicz, Małgorzata Józkowiak, Magdalena Kulus, Jakub Kulus, Michał Gnus, Dominika Domagała, Piotr Paweł Chmielewski, Anna Kałuża and 10 more

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Krzysztof DataDivision of Anatomy, Department of Human Morphology and Embryology, Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland.ORCID 0000-0002-9018-5357
Wiesława KrancDepartment of Anatomy, Poznan University of Medical Sciences, 60-781 Poznan, Poland.ORCID 0000-0001-7518-4834
Małgorzata BlatkiewiczDepartment of Histology and Embryology, Poznan University of Medical Sciences, 60-812 Poznan, Poland.ORCID 0000-0001-5506-2397
Małgorzata JózkowiakDepartment of Toxicology, Poznan University of Medical Sciences, 60-812 Poznan, Poland.
Magdalena KulusDepartment of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland.ORCID 0000-0003-1098-5627
Jakub KulusDepartment of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland.ORCID 0000-0003-0694-8006
Michał GnusDepartment of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland.
Dominika DomagałaDivision of Anatomy, Department of Human Morphology and Embryology, Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland.ORCID 0009-0008-5220-7851
Piotr Paweł ChmielewskiDivision of Anatomy, Department of Human Morphology and Embryology, Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland.ORCID 0000-0002-6995-123X
Anna KałużaDepartment of Biochemistry and Immunochemistry, Division of Chemistry and Immunochemistry, Wroclaw Medical University, 50-369 Wroclaw, Poland.ORCID 0000-0003-0161-3012
Agnieszka ŻokDivision of Philosophy of Medicine and Bioethics, Poznan University of Medical Sciences, 60-812 Poznan, Poland.ORCID 0000-0002-5560-5432
Julia NieboraDivision of Anatomy, Department of Human Morphology and Embryology, Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Artur BryjaDivision of Anatomy, Department of Human Morphology and Embryology, Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Anna OlechnowiczDepartment of Histology and Embryology, Poznan University of Medical Sciences, 60-812 Poznan, Poland.ORCID 0000-0001-6043-5637
Hanna Piotrowska-KempistyDepartment of Toxicology, Poznan University of Medical Sciences, 60-812 Poznan, Poland.
Paul MozdziakPrestage Department of Poultry Science, North Carolina State University, Raleigh, NC 27695, USA.ORCID 0000-0002-1575-3123
Bartosz KempistyDivision of Anatomy, Department of Human Morphology and Embryology, Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Paweł AntosikDepartment of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland.
Dorota BukowskaDepartment of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland.
Mariusz T SkowrońskiDepartment of Basic and Preclinical Science, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland.ORCID 0000-0002-9826-2345

Funding

National Science Centre WNBiW/Opus19/2020/37/B/NZ5/03926
6 · The paper itself

Abstract

Granulosa cells (GCs), an element of the ovarian follicle, are crucial for oocyte maturation, folliculogenesis, and steroidogenesis. Granulosa cells play a crucial role in fertilization by providing metabolic and hormonal support to the oocyte, maintaining its quality and regulating its meiotic arrest. Oocyte quality and fertilization efficiency depend on the proper activity of GCs, especially their mutual communication, providing metabolic support and protecting against oxidative stress. When interrupted, they may take part in the pathogenesis of polycystic ovary syndrome, premature ovarian failure, primary ovarian insufficiency, and diminished ovarian reserve. GCs are enclosed in the antrum where they communicate with surrounding cells, create a dynamic microenvironment, and regulate hormone biosynthesis. To analyze molecular mechanisms regulating endogenous signaling, it is important to consider the dynamic transcriptomic response of porcine GCs during in vitro culturing over 48, 96, and 144 h. Transcriptomic analysis revealed a variable and dynamic transcriptional upregulation of genes associated with cellular response to endogenous and external stimuli, chemical compound metabolism, vascular development, and GCs migration. Also, proven by Gene Ontology (GO) enrichment analysis, the following terms were highlighted: "cellular response to chemical stimulus" and "cellular response to organic substance". Specific genes, such as

Indexed as

Granulosa CellsTranscriptomeAnimalsCells, CulturedFemaleGene Expression ProfilingGene Expression RegulationOvarian FollicleSignal TransductionSwinefolliculogenesisintercellular communicationovarian folliclesignaling pathwaysteroidogenesis

Identifiers

PMID42353161
PMCPMC13300499

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.