Evidence mapPaperPMID 42353632Full record

ReviewCurrent issues in molecular biology2026

MALDI-TOF Mass Spectrometry for Glioblastoma Secretome Biomarker Screening: A Review of Challenges and Perspectives.

David Aebisher, Klaudia Dynarowicz, Rostyslav Marunych, Izabela Rudy, Kacper Rogóż, Aleksandra Kawczyk-Krupka, Piotr Oleś, Dorota Bartusik-Aebisher

Abstract readReview
In one paragraph

Review in Current issues in molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

David AebisherDepartment of Photomedicine and Physical Chemistry, Faculty of Medicine, University of Rzeszów, 35-959 Rzeszów, Poland.ORCID 0000-0002-2661-6570
Klaudia DynarowiczDepartment of Biochemistry and General Chemistry, Faculty of Medicine, University of Rzeszów, 35-959 Rzeszów, Poland.ORCID 0000-0001-6851-5869
Rostyslav MarunychDoctoral School, Faculty of Medicine, University of Rzeszów, 35-310 Rzeszów, Poland.
Izabela RudyStudent Scientific Club of Biochemists URCell, Faculty of Medicine, University of Rzeszów, 35-959 Rzeszów, Poland.
Kacper RogóżStudent Scientific Club of Biochemists URCell, Faculty of Medicine, University of Rzeszów, 35-959 Rzeszów, Poland.ORCID 0009-0002-7999-3449
Aleksandra Kawczyk-KrupkaDepartment of Internal Diseases, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, Medical University of Silesia, Batorego 15, 41-902 Bytom, Poland.ORCID 0000-0003-1284-3809
Piotr OleśDepartment of Internal Diseases, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, Medical University of Silesia, Batorego 15, 41-902 Bytom, Poland.
Dorota Bartusik-AebisherDepartment of Biochemistry and General Chemistry, Faculty of Medicine, University of Rzeszów, 35-959 Rzeszów, Poland.ORCID 0000-0002-5557-5464

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Glioblastoma (GBM) remains one of the most aggressive malignancies, characterized by profound heterogeneity and a dismal prognosis. While genomic and transcriptomic profiling have provided structural insights, they often fail to capture the dynamic interactions within the tumor microenvironment (TME). Secretome analysis-the study of proteins actively secreted by tumor cells-offers a functional readout of these interactions and a reservoir for potential biomarkers. In this review, we critically evaluate the role of MALDI-TOF Mass Spectrometry as a strategic tool for GBM secretome profiling. While Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) remains the gold standard for deep protein discovery, we argue that MALDI-TOF's speed, cost-effectiveness, and high-throughput capabilities position it as an ideal platform for clinical screening and "spectral phenotyping." We discuss the technical hurdles, such as ion suppression and the "leakome" (intracellular contamination), and highlight how integrating MALDI with Extracellular Vesicle (EV) enrichment and Artificial Intelligence (AI) can bridge the gap between in vitro discovery and clinical application.

Indexed as

cell linesextracellular vesiclesglioblastomaMALDIMALDI-TOFproteomicssecreted proteinssecretome

Identifiers

PMID42353632
PMCPMC13297980

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.