ArticleMolecular nutrition & food research2026
Culture Optimization of the IPEC-J2 Piglet Jejunum Cell Line for Applications in Infant Nutrition Research.
Article in Molecular nutrition & food research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The intestinal porcine epithelial cell line (IPEC-J2) is a nontransformed jejunal model derived from unsuckled piglets. It is increasingly used in gut physiology research, yet its application to nutrient absorption remains underexplored. Four media types were assessed for their ability to support IPEC-J2 barrier maturation and permeability: Dulbecco's modified Eagle medium/Ham's F-12 with 10% fetal bovine serum (FBS10), 10% porcine serum (PS10), 5% porcine serum (PS5) supplemented with Wnt3a-R-spondin-Noggin conditioned medium (PS5+L-WRN), and PS5 with epidermal growth factor and insulin-transferrin-selenium (PS5+EGF+ITS). Culture medium influenced epithelial phenotype, with more than a 30-fold range in transepithelial electrical resistance (TEER), which plateaued by day 12. PS10 supported a balanced profile, characterized by intermediate TEER (2395.00 ± 485.51 Ω × cm
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