Evidence map›Paper›PMID 42358650›Full record

ArticleStem cells international2026

Efficient Derivation and Transcriptional Characterization of Mouse Extra-Embryonic Endoderm Stem Cell Lines Generated by Somatic Cell Nuclear Transfer.

Shuaipeng Li, Shu Wei, Guomeng Li, Mei Hu, Jiangwei Lin, Wandong Bao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells international, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Shuaipeng LiDepartment of Laboratory Animal Science, Kunming Medical University, Kunming, 650500, Yunnan, China, kmmc.cn.
Shu WeiState Key Laboratory of Genetic Evolution and Animal Models, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Yunnan, China, cas.cn.
Guomeng LiState Key Laboratory of Genetic Evolution and Animal Models, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Yunnan, China, cas.cn.
Mei HuState Key Laboratory of Genetic Evolution and Animal Models, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Yunnan, China, cas.cn.
Jiangwei LinDepartment of Laboratory Animal Science, Kunming Medical University, Kunming, 650500, Yunnan, China, kmmc.cn.ORCID https://orcid.org/0000-0002-1307-3079
Wandong BaoDepartment of Laboratory Animal Science, Kunming Medical University, Kunming, 650500, Yunnan, China, kmmc.cn.ORCID https://orcid.org/0000-0002-3428-0966

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Somatic cell nuclear transfer (SCNT) holds great promise for regenerative medicine and agriculture, but its application is severely hampered by low efficiency, primarily attributable to aberrant epigenetic reprogramming. Although embryonic stem cells (ESCs) and trophoblast stem cells (TSCs) have been successfully derived from cloned embryos, an in vitro counterpart of the primitive endoderm (PrE) lineage has remained unavailable. To address this gap, this study reports the first successful establishment of extra-embryonic endoderm stem cell lines (XENs) from mouse SCNT-derived blastocysts (NT-XENs). Under conventional culture conditions, NT-XENs were generated from hybrid B6D2F1 blastocysts at a high efficiency of 55%, statistically comparable to that of fertilization-derived XEN lines (FD-XENs, 50%), whereas derivation from inbred C57BL/6J SCNT-derived blastocysts was markedly lower (12.5%). Immunofluorescence and NanoString multiplex gene expression profiling confirmed that NT-XENs robustly expressed specific marker genes for PrE/XENs (e.g.,

Indexed as

aberrant epigenetic reprogrammingextra-embryonic endoderm stem cell lineimprinting errorsNanoString multiplex gene expression profilingprimitive endodermsomatic cell nuclear transfer

Identifiers

PMID42358650
PMCPMC13291796

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.