ArticlePhytochemical analysis : PCA2026
Integrating 3D Chromatography With Orbitrap Mass Spectrometry to Profile the Chemical Constituents in JTTZ Formula and Investigate Plasma Pharmacokinetic Changes in Obese Rats.
Article in Phytochemical analysis : PCA, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
introductionChemical profiling and exposure of multicomponent Chinese medicine formula (CMF) are essential for understanding the multitarget treatment mechanism, as they consist of bioactive substances with varying polarities, molecular weights, and charges. However, the chemical composition of CMF is highly intricate, primarily due to the presence of multiple herbs, rendering chemical identification a challenging task.
objectiveJiangtang Tiaozhi formula (JTTZ), commonly used for early diabetes intervention, was chosen to validate our standardized analytical strategy integrating offline three - dimensional (3D) chromatography-DDA (data-dependent acquisition) with a precursor ion list (PIL)-comparative pharmacokinetic study. MATERIALS AND
methodsWe have implemented an offline 3D chromatography coupled with data-dependent MS2 acquisition, including PIL, to obtain comprehensive compound information within JTTZ. We then utilized the MRM quantification approach to determine the pharmacokinetics of the 13 major circulating constituents identified in JTTZ. Finally, the variations in pharmacokinetic parameters between high-fat diet (HFD) and normal chow diet (NCD) rat models are further illustrated by comparative pharmacokinetics.
resultUsing an offline 3D chromatography platform, 186 compounds were identified in JTTZ, as opposed to the 68 compounds detected with classical one-dimensional (1D) LC-MS technology. Through the chemical profiling results of the multidimensional chromatography, we found 41 components of JTTZ absorbed in plasma. We then utilized the MRM quantification approach to determine the pharmacokinetics of the 13 major circulating constituents identified in JTTZ. Finally, the variations in pharmacokinetic parameters between HFD and normal chow diet (NCD) rat models are further illustrated by comparative pharmacokinetics. In the HFD group, the T
conclusionThe results show that the pathological state caused by a HFD changed the plasma pharmacokinetics of jatrorrhizine, mangiferin, and timosaponin BII in JTTZ, providing drug exposure evidence for the involvement of JTTZ in early diabetes intervention. The stepwise pipeline for the "offline 3D chromatography-DDA acquisition method with PIL-comparative pharmacokinetic study" in the case study of JTTZ has the potential to reveal the exposure profile of other CMFs, from compound identification to pharmacokinetic analysis.
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