Evidence map›Paper›PMID 42366338›Full record

ArticleBMC complementary medicine and therapies2026

Isolation and identification of hasubanan alkaloids having anti-cholinesterase and antioxidant activity from the stem Stephania japonica.

Anik Kumar Dey, Md Yusuf Al-Amin, Rafia Ferdous, A H M Khurshid Alam, Aziz Abdur Rahman, Md Bayazid Hossen, Md Nurul Haque Mollah, Md Golam Sadik

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Article in BMC complementary medicine and therapies, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Anik Kumar DeyDepartment of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh.
Md Yusuf Al-AminDepartment of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh.
Rafia FerdousDepartment of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh.
A H M Khurshid AlamDepartment of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh.
Aziz Abdur RahmanDepartment of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh.
Md Bayazid HossenBioinformatics Lab, Department of Statistics, University of Rajshahi, Rajshahi, 6205, Bangladesh.
Md Nurul Haque MollahBioinformatics Lab, Department of Statistics, University of Rajshahi, Rajshahi, 6205, Bangladesh.
Md Golam SadikDepartment of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh. gsadik2@ru.ac.bd.

Funding

University Grants Commission of Bangladesh 37-01-0000-073-04-012/2019
6 · The paper itself

Abstract

backgroundA recent report showed that Stephania japonica chloroform fraction has potential anticholinesterase and antioxidant activities and is able to improve learning and memory in mice. Therefore, the aim of the present study was to isolate and identify compounds from the chloroform fraction with cholinesterase inhibitory and antioxidant activity that may be useful as new candidates for the treatment of AD.

methodsChromatographic methods were used for isolation of compounds and the isolated compounds were analyzed by spectroscopic methods for structure elucidation. Acetyl- and butyryl-cholinesterase inhibitory activity were evaluated for by Ellman's method and the antioxidant activity by several in vitro models such as DPPH and hydroxyl radicals scavenging, reducing power, total antioxidant activity, and inhibition of brain lipid peroxidation. The interaction of cholinesterase enzymes and isolated compounds were examined by molecular docking studies.

resultsBioactivity guided approach led to the isolation of four compounds from the chloroform fraction and identified as aknadinine, aknadilactam, aknadicine and stephisoferuline on the basis of their

conclusionThe hasubabanan alkaloids isolated from S. japonica may represent a new class of anti-cholinesterase compounds. The multitargeted activity of hasubanan alkaloids may lead to new candidates for the treatment of AD.

Indexed as

AlkaloidsAntioxidantsCholinesterase InhibitorsPlant ExtractsStephaniaAcetylcholinesteraseAnimalsMolecular Docking SimulationAcetylcholinesteraseAlkaloidsAntioxidantsCholinesterase InhibitorsPlant ExtractsAlzheimer’s diseaseAnti-cholinesteraseAntioxidantHasubananMolecular dockingStephania japonica

Identifiers

PMID42366338
PMCPMC13573421

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.