Evidence map›Paper›PMID 42369404›Full record

ArticleNAM journal2025

Development and characterization of a standardized adipogenesis assay for testing metabolism disrupting chemicals using human bone marrow derived mesenchymal stem cells.

Xiao-Min Ren, Richard C Chang, Angélica Amorim Amato, Yikai Huang, Brittanie Yukimtiao, Alexandra Esser, Emma Witteveen, Juliette Legler, Jorke H Kamstra, Bruce Blumberg

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in NAM journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xiao-Min RenDepartment of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.
Richard C ChangDepartment of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.
Angélica Amorim AmatoDepartment of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.
Yikai HuangDepartment of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.
Brittanie YukimtiaoDepartment of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.
Alexandra EsserDivision of Toxicology, Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Emma WitteveenDivision of Toxicology, Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Juliette LeglerDivision of Toxicology, Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Jorke H KamstraDivision of Toxicology, Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Bruce BlumbergDepartment of Developmental and Cell Biology, University of California, Irvine, CA 92697-2300, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Obesity is a global health concern linked to various diseases. Recently, chemicals known as "obesogens" have been identified as significant contributors to obesity, highlighting the need for reliable methods to screen chemicals for their obesity-promoting potential. This study aimed to develop a standardized adipogenesis assay using primary human bone marrow-derived multipotent mesenchymal stromal stem cells (hBM-MSCs) to evaluate the adipogenic potential of chemicals. Lipid accumulation in hBM-MSCs was measured after chemical exposure. Assay conditions including cell confluency, fluorescent staining of neutral lipids and fluorometric detection were optimized and standardized. Using the established method, we evaluated the effects of six selected chemicals on the adipogenic responses of hBM-MSCs from different donors, including an in-house generated immortalized hBM-MSC cell line. Not all donor-derived hBM-MSCs were shown to be suitable for the assay, but we observed comparable results among suitable hBM-MSCs and between primary hBM-MSCs and their immortalized derivative cell line. Comparisons with the well-established 3T3-L1 preadipocyte model revealed a strong correlation with the hBM-MSCs assay. We observed that experimental details such as cell density, fluorescent measurement and cell donors can greatly affect outcomes; this stresses the importance of a well described standard operating procedure (SOP). Our developed test method, including a detailed SOP, paves the way for a validated test methodology that is urgently needed and currently lacking for the identification of obesogenic endocrine disruptors.

Indexed as

Adipogenesis assayImmortalized hBM-MSC cell lineMesenchymal stromal stem cellsMetabolism disrupting chemicalsObesity

Identifiers

PMID42369404
PMCPMC13288643

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.