Evidence map›Paper›PMID 42371642›Full record

ArticleVeterinary medicine and science2026

Genomic Insights Into Antimicrobial Resistance and Virulence of Enterococcus avium Strains From Bovine Mastitis in Some Selected Dairy Farms of Bangladesh.

Monira Rahaman, Md Morshedur Rahman, Kh Yeashir Arafat, Md Abu Ahsan Gilman, Naim Siddique, Taniya Sultana, Ziban Chandra Das, Anup Kumar Talukder, Tofazzal Islam, A N M Aminoor Rahman and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary medicine and science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Monira RahamanMolecular Biology and Bioinformatics Laboratory, Department of Gynaecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, Bangladesh.
Md Morshedur RahmanMolecular Biology and Bioinformatics Laboratory, Department of Gynaecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, Bangladesh.
Kh Yeashir ArafatMolecular Biology and Bioinformatics Laboratory, Department of Gynaecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, Bangladesh.
Md Abu Ahsan GilmanMolecular Biology and Bioinformatics Laboratory, Department of Gynaecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, Bangladesh.
Naim SiddiqueMolecular Biology and Bioinformatics Laboratory, Department of Gynaecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, Bangladesh.ORCID https://orcid.org/0009-0003-5710-7511
Taniya SultanaMolecular Biology and Bioinformatics Laboratory, Department of Gynaecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, Bangladesh.
Ziban Chandra DasMolecular Biology and Bioinformatics Laboratory, Department of Gynaecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, Bangladesh.
Anup Kumar TalukderMolecular Biology and Bioinformatics Laboratory, Department of Gynaecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, Bangladesh.
Tofazzal IslamInstitute of Biotechnology and Genetic Engineering, Gazipur Agricultural University, Gazipur, Bangladesh.
A N M Aminoor RahmanMolecular Biology and Bioinformatics Laboratory, Department of Gynaecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, Bangladesh.
M Nazmul HoqueMolecular Biology and Bioinformatics Laboratory, Department of Gynaecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, Bangladesh.ORCID https://orcid.org/0000-0002-4861-0030

Funding

from the Bangladesh Bureau of Educational Information and Statistics
6 · The paper itself

Abstract

backgroundBovine mastitis remains a major global threat to dairy production and animal welfare, with the increasing emergence of multidrug-resistant (MDR) pathogens severely undermining the efficacy of conventional antimicrobial therapies and complicating disease control strategies. Enterococcus avium, traditionally understudied in livestock, has been occasionally associated with mastitis and poses potential zoonotic and antimicrobial resistance (AMR) risks.

objectivesThis study aimed to investigate the prevalence, AMR, virulence repertoire and genomic features of E. avium isolates from milk, faeces and soil in some selected dairy farms with mastitis in Bangladesh.

methodsA total of 110 samples (milk, faecal and soil) were collected and screened for E. avium using selective culture and polymerase chain reaction (PCR). Antimicrobial susceptibility was determined against 15 antibiotics. Four MDR E. avium isolates (4M1, 4S1, 4F1 and 4F2) were selected for whole-genome sequencing (WGS) to characterize their genomic diversity, functional potential, resistome and virulome in dairy cows and associated environments.

resultsE. avium was detected in 56.36% of samples, with highest prevalence in milk (47.8%). MDR was highly prevalent (93.5%), with frequent resistance to sulphonamides, nitrofurantoin and oxacillin, whereas gentamicin retained activity. Genomic analyses revealed conserved core genomes alongside variable accessory elements, indicating both evolutionary stability and adaptive potential. Phylogenetic proximity to human-derived strains highlights zoonotic risk. Functional profiling demonstrated robust metabolism, environmental sensing, adhesion-related virulence factors and multiple bacteriocin clusters, supporting persistence and microbial competition. ARGs conferring multidrug efflux, β-lactam and fluoroquinolone resistance were conserved across isolates.

conclusionsE. avium is a prevalent mastitis pathogen, highly conserved among the studied isolates, with significant virulence and AMR profiles, highlighting its zoonotic potential and need for One Health-based surveillance strategies.

Indexed as

Anti-Bacterial AgentsDrug Resistance, BacterialDrug Resistance, Multiple, BacterialEnterococcusGenome, BacterialGram-Positive Bacterial InfectionsMastitis, BovineAnimalsBangladeshCattleDairyingFemalePrevalenceVirulenceAnti-Bacterial Agentsbovine mastitisenterococcus aviummultidrug resistanceone healthvirulence factors

Identifiers

PMID42371642
PMCPMC13312985

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.