Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Wenxia Lin, Omar Herrera-Asmat, Alexander B Tong, Tiantian Kong, Carlos Bustamante

Abstract read

In one paragraph

Article in Methods (San Diego, Calif.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

Update of
Single-Molecule Methods to Investigate Mechanisms of Transcription by RNA Polymerase of2026

5 · Who and what money

Authors and funding

5 authors.

Wenxia LinDepartment of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, Guangdong 518000, China; Institute for Quantitative Biosciences-QB3, University of California, Berkeley, CA 94720, United States.

Omar Herrera-AsmatInstitute for Quantitative Biosciences-QB3, University of California, Berkeley, CA 94720, United States; Department of Molecular Cell and Biology, University of California, Berkeley, CA 94720, United States; Health Sciences Research Institute, University of California, Merced, CA 95343, United States.

Alexander B TongInstitute for Quantitative Biosciences-QB3, University of California, Berkeley, CA 94720, United States; Department of Chemistry, University of California, Berkeley, CA 94720, United States.

Tiantian KongDepartment of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, Guangdong 518000, China. Electronic address: ttkong@szu.edu.cn.

Carlos BustamanteInstitute for Quantitative Biosciences-QB3, University of California, Berkeley, CA 94720, United States; Department of Molecular Cell and Biology, University of California, Berkeley, CA 94720, United States; Department of Chemistry, University of California, Berkeley, CA 94720, United States; Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, United States; Department of Physics, University of California, Berkeley, CA 94720, United States; Jason L Choy Laboratory of Single-Molecule Biophysics, University of California, Berkeley, CA 94720, United States; Biophysics Graduate Group, University of California, Berkeley, CA 94720, United States; Kavli Energy Nanoscience Institute, University of California, Berkeley, CA 94720, United States. Electronic address: carlosb@berkeley.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single molecule methods have become prevalent tools in elucidating molecular processes across various life science fields over the past three decades, driving breakthroughs in understanding their underlying molecular mechanisms. In our study, we employed two single-molecule methods, Förster Resonance Energy Transfer (smFRET) and high-resolution optical tweezers, to investigate the transcription of Mycobacterium tuberculosis RNA polymerase (MtbRNAP) from initiation through to termination. We aim to provide a set of comprehensive biophysical tools to deepen our current understanding of MtbRNAP and its transcription factors. These experimental assays represent an important step towards unraveling the molecular dynamics and interactions that support transcription in Mycobacterium tuberculosis.

Indexed as

Bacterial ProteinsDNA-Directed RNA PolymerasesFluorescence Resonance Energy TransferMycobacterium tuberculosisSingle Molecule ImagingTranscription, GeneticOptical TweezersBacterial ProteinsDNA-Directed RNA PolymerasesMtbCarDMtbGreAMycobacterium tuberculosis RNA polymeraseOptical tweezersRNA co-transcriptional foldingsmFRETTranscription elongationTranscription initiationTranscription termination

Identifiers

PMID42372956

DOI10.1016/j.ymeth.2026.06.008

What Socratic holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.