ArticleBMC plant biology2026
Long non-coding RNA landscape and ceRNA networks underlying the drought response of Cannabis sativa L.
Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundDrought is a major constraint on plant productivity, and long non-coding RNAs (lncRNAs) are increasingly recognized as key regulators of plant stress responses. Yet drought-responsive lncRNAs remain largely uncharacterized in Cannabis sativa L., a species of growing economic and medicinal importance.
methodsWe combined RNA-seq analysis of C. sativa leaves under progressive drought stress with physiological monitoring and independent qRT-PCR validation. A stringent identification pipeline integrating StringTie assembly with CPC2 and Pfam coding-potential filtering was applied, followed by differential expression analysis (DESeq2), cis-target prediction, functional enrichment, ceRNA network construction, and transcription-factor profiling.
resultsThe pipeline yielded 2,096 high-confidence novel lncRNAs with the canonical structural signatures of plant lncRNAs - shorter length, simpler exonic architecture, and lower steady-state expression than mRNAs. Differential expression analysis identified 51 high-confidence differentially expressed lncRNAs and 575 differentially expressed mRNAs in response to drought. Functional enrichment of predicted lncRNA cis-targets highlighted phenylpropanoid and lignin biosynthesis, abscisic acid signaling, and vacuolar ion transport as dominant themes. Transcription-factor profiling revealed a striking over-representation of FAR1-family regulators among lncRNA targets. A DEL-anchored ceRNA sub-network further uncovered two candidate regulatory modules, both showing directionally concordant qRT-PCR expression profiles.
conclusionsThese results provide the first systematic characterisation of polyA-selected, leaf-expressed drought-responsive lncRNAs in C. sativa, propose a hypothesis of lncRNA-mediated light-drought crosstalk via FAR1/FHY3 regulators, requiring future functional validation, and nominate candidate regulatory nodes for future functional studies and stress-resilience breeding.
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