Evidence map›Paper›PMID 42374805›Full record

ArticleTransboundary and emerging diseases2026

Development and Application of a Blocking ELISA for the Detection of Feline Calicivirus Antibodies Based on Monoclonal Antibodies Against VP1 Protein.

Haojie Wang, Lihong Xue, Bowen Shan, Shuyan Wu, Tongqin An, Changqing Yu, Changyou Xia, He Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transboundary and emerging diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Haojie WangState Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China, caas.cn.ORCID https://orcid.org/0009-0007-2213-8229
Lihong XueState Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China, caas.cn.
Bowen ShanState Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China, caas.cn.
Shuyan WuState Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China, caas.cn.
Tongqin AnState Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China, caas.cn.
Changqing YuSchool of Advanced Agricultural Sciences, Yibin Vocational and Technical College, Yibin, Sichuan, China.ORCID https://orcid.org/0000-0002-9548-7151
Changyou XiaState Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China, caas.cn.ORCID https://orcid.org/0000-0002-2701-1143
He ZhangState Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China, caas.cn.ORCID https://orcid.org/0009-0002-8296-9730

Funding

Heilongjiang Province Natural Fund Joint Guidance Project LH2024C059National Key Research and Development Program of China 2023YFF0724603National Key Research and Development Program of China 2023YFF0724604National Natural Science Foundation of China-Youth Science Fund 32500449Prevention and Control of Emerging and Major Infectious Diseases-National Science and Technology Major Project 2025ZD01900703
6 · The paper itself

Abstract

In this study, nine monoclonal antibodies (mAbs) against the FCV VP1 protein were successfully prepared using traditional hybridoma technology, and all of them could simultaneously recognize linear and conformational epitopes. Their subtypes mainly included IgG1/kappa, IgG2a/kappa, IgG1/lambda, IgG2b/kappa, IgG2b/lambda, and IgM/kappa. Based on the mAb 8F9 with the highest blocking activity, a blocking enzyme-linked immunosorbent assay (ELISA) method for detecting cat calicivirus antibodies was established. After systematic optimization, the antigen coating concentration was determined to be 1.0 μg/mL, the serum dilution was 1:8, and the dilution of horseradish peroxidase (HRP)-labeled mAb was 1:400. The critical value for detecting cat serum was 23.5%, the sensitivity was 94.9%, and the specificity was 93.02%. There was no cross-reaction with common pathogens such as feline parvovirus and feline mycoplasma. The repeatability was good, with intra-batch and inter-batch coefficients of variation (CV) <10%. Clinical validation showed that the total coincidence rate with the indirect ELISA method recommended in the local standard was 96.19% (kappa = 0.9). The sensitive and specific blocking ELISA method established in this study can provide reliable technical support for FCV antibody screening, vaccine immune evaluation, and specific pathogen-free (SPF) cat breeding.

Indexed as

Antibodies, MonoclonalAntibodies, ViralCaliciviridae InfectionsCalicivirus, FelineCapsid ProteinsCat DiseasesAnimalsCatsEnzyme-Linked Immunosorbent AssaySensitivity and SpecificityAntibodies, MonoclonalAntibodies, ViralCapsid Proteinsantibody detectionblocking ELISAfeline calicivirusmonoclonal antibodyvaccine evaluationVP1 protein

Identifiers

PMID42374805
PMCPMC13315827

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.