Evidence map›Paper›PMID 42374836›Full record

ArticleTransboundary and emerging diseases2026

The PCK2-Traf6-Tollip Axis Restricts PEDV Replication by Orchestrating Selective Autophagic Degradation of the Viral Nucleocapsid Protein.

Ao Gao, Xinyu Yang, Wenzhen Qin, Dongfang Zheng, Yuchang Liu, He Sun, Zongyan Chen, Guangzhi Tong, Shuang Xue, Ning Kong and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transboundary and emerging diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ao GaoCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China, henau.edu.cn.ORCID https://orcid.org/0009-0001-9722-3918
Xinyu YangShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China, caas.cn.ORCID https://orcid.org/0009-0009-4808-9714
Wenzhen QinShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China, caas.cn.ORCID https://orcid.org/0009-0005-7497-0362
Dongfang ZhengShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China, caas.cn.ORCID https://orcid.org/0009-0002-7539-9717
Yuchang LiuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China, caas.cn.ORCID https://orcid.org/0009-0001-1662-8017
He SunShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China, caas.cn.ORCID https://orcid.org/0009-0004-0989-4765
Zongyan ChenShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China, caas.cn.ORCID https://orcid.org/0009-0005-0830-912X
Guangzhi TongShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China, caas.cn.ORCID https://orcid.org/0000-0001-7048-0837
Shuang XueZhumadian Animal Disease Control and Inspection Center, Zhumadian, Henan, China.ORCID https://orcid.org/0009-0000-0047-7165
Ning KongShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China, caas.cn.ORCID https://orcid.org/0000-0001-7264-9069
Lanlan ZhengCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China, henau.edu.cn.ORCID https://orcid.org/0000-0001-8284-9152

Funding

National Natural Science Foundation of China 32302891Outstanding Young Innovation Research Group Project of Natural Science Foundation of Henan Province 262300421002Science and Technology Innovation Leading Talent Support Program of Henan Province 264200510045Shanghai Agricultural Science and Technology Innovation Program K2024001Young Top-Notch Talents Foundation of Henan Agricultural University
6 · The paper itself

Abstract

Porcine epidemic diarrhea virus (PEDV) is a devastating enteric pathogen in neonatal piglets, causing outbreaks with high mortality. The lack of effective vaccines or treatments makes elucidating host-pathogen interactions essential for developing control strategies. In this study, we identify phosphoenolpyruvate carboxykinase 2 (PCK2) as a host restriction factor that targets the viral nucleocapsid (N) protein for degradation, thereby suppressing PEDV replication. This PCK2-mediated antiviral effect was reversed by autophagy inhibitors, indicating the involvement of a selective autophagic. Mechanistically, we found that PCK2, the N protein, the E3 ubiquitin ligase Traf6, and the cargo receptor Tollip form a functional complex. Depletion of either Traf6 or Tollip disrupted the autophagy pathway, restored N protein stability, and consequently rescued viral replication from PCK2 inhibition. This study unveils a novel antiviral mechanism in which PCK2 orchestrates the selective autophagic degradation of the PEDV N protein, highlighting the PCK2-Traf6-Tollip axis as a promising therapeutic direction.

Indexed as

AutophagyCoronavirus InfectionsNucleocapsid ProteinsPorcine epidemic diarrhea virusTNF Receptor-Associated Factor 6Virus ReplicationAnimalsHost-Pathogen InteractionsSwineNucleocapsid ProteinsTNF Receptor-Associated Factor 6autophagy pathwaydegradationPCK2PEDV N

Identifiers

PMID42374836
PMCPMC13315828

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.