ArticleOpen veterinary journal2026
Real time PCR identification of
Article in Open veterinary journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Authors and funding
2 authors.
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Abstract
Aim: The objective of this article was to employ morphological identification and molecular techniques to detect and confirm Methods: A study of a total of 100 imported domestic cats from importers, which were brought to the veterinary clinic for regular check-ups. Under a stereomicroscope, ticks were identified by their morphology and using standard taxonomic keys. DNA obtained from every individual tick sampled was examined by targeting tick-specific gene markers through conventional PCR. Real-time PCR detects and confirms the types of tick species and their associated pathogens in their natural habitats. Results: The results of morphological identification revealed the presence of the tick in 18 out of 100 cats (18%), exhibiting the morphological characteristics of the Conclusion: The real-time PCR method was able to detect Ixodes holocyclus ticks with speed, accuracy, and 100% specificity. Ticks can infest cats, allowing them to carry these parasites into both the house and the environment. This also raises the risk of transmission, including zoonotic transmission. Furthermore, this can also pose a public health hazard.
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