ArticleOpen veterinary journal2026
Molecular weight profile of sperm proteins and their association with Kinematic motility in different buck breeds.
Article in Open veterinary journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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6 authors.
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Abstract
Background: Reproductive efficiency in bucks is a critical determinant of genetic improvement and livestock development programs, particularly in smallholder farming systems where bucks contribute substantially to household income and food security. Aim: This study aimed to characterize the molecular weight profiles of sperm proteins and their association with kinematic motility parameters in frozen-thawed semen from Anglonubian, Ettawa Cross, Boer, and Saanen bucks. Method: Frozen semen samples were collected from 16 bucks representing the 4 breeds. Sperm motility and kinematic parameters, including total motility, progressive motility, velocity parameters (VAP, velocity, VSL), linearity (LIN), straightness (STR), amplitude of lateral head displacement (ALH), and beat cross frequency, were analyzed using computer-assisted semen analysis. Sperm proteins were extracted, quantified, and separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine their molecular weight profiles. ANOVA and Pearson correlation tests were used in this study. Results: All breeds produced frozen-thawed semen with acceptable motility parameters for artificial insemination. Boer bucks exhibited significantly lower motility than Ettawa Cross and Saanen breeds ( Conclusion: Variations in sperm protein molecular weight profiles and kinematic motility were evident among buck breeds, suggesting the presence of potential breed-specific markers. These findings provide a foundation for the identification of molecular determinants of fertility and support the development of selection strategies for superior sires in buck breeding programs.
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