Hyperhomocysteinemia promotes lipid deposition in skeletal muscle.

Background: Lipid storage myopathy (LSM) is characterized by abnormal lipid accumulation in skeletal muscle. Emerging evidence suggests that environmental factors, including the use of antidepressants such as sertraline, may trigger LSM. Given the established link between hyperhomocysteinemia (HHcy) and disrupted lipid metabolism, we investigated its potential role in skeletal muscle lipid deposition. Methods: We enrolled six patients with HHcy undergoing muscle biopsy and explored their clinical and pathological characteristics of skeletal muscle. The mechanistic link was explored in muscle tissues from patients through transcriptomic profiling, quantitative real-time polymerase chain reaction (qRT-PCR), western blotting, and enzymatic assays, and validated in C2C12 myotubes. Results: Four of the six patients presented with clinical myopathic manifestations, including progressive muscle weakness and exercise intolerance, which resolved completely after B-vitamin supplementation, while abnormal skeletal muscle lipid deposition was observed in all six patients. Transcriptome and qRT-PCR analyses demonstrated a significant upregulation of the acetyl-CoA carboxylase β ( Conclusion: This study indicates that HHcy is closely associated with abnormal skeletal muscle lipid deposition. HHcy may correlate with increased ACC2 expression, which elevates malonyl-CoA levels. This, in turn, suppresses CPT1 activity and facilitates abnormal lipid accumulation in skeletal muscle.