ArticleJournal of extracellular biology2026
Characterisation and Comparative Evaluation of miRNA Contained in Small Extracellular Vesicles Isolated From Bovine Milk and Whey.
Article in Journal of extracellular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Small extracellular vesicles (EVs) from bovine milk and whey are emerging as biologically active carriers of microRNA (miRNA), yet their composition and variability among different dairy sources remain incompletely understood. We conducted an integrated characterisation of EV-associated miRNAs isolated from milk and whey collected from three dairy farms. EVs were purified and validated according to MISEV2023 criteria, and small RNA sequencing was performed on milk and whey samples collected on three different days at each farm (18 samples). After quality filtering and adaptor trimming, mapped reads were normalised to counts per million (CPM). To ensure robust detection, only miRNAs present in at least 67% of samples were retained, resulting in a high-confidence set of 329 miRNAs for downstream analyses. Milk-derived EVs showed a higher overall miRNA read count than whey-derived EVs (9.45 ± 5.3 million vs. 5.03 ± 2.5 million reads;
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