Evidence map›Paper›PMID 42384491›Full record

ArticleSTAR protocols2026

Protocol for quantitative live-cell imaging of early GPCR trafficking using acid-stable afCFP-Venus FRET.

Yu Guo, Toshimasa Takahashi, Cheng Wang, Lisha Yi, Daisuke Nawa, Nanxiang Yin, Weidong Liu, Ziwei Wang, Heedong Jeon, Akitoshi Hara and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yu GuoDepartment of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Toshimasa TakahashiDepartment of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan; Department of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada. Electronic address: toshimasa.takahashi@geriat.med.osaka-u.ac.jp.
Cheng WangDepartment of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Lisha YiDepartment of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Daisuke NawaDepartment of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Nanxiang YinDepartment of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Weidong LiuDepartment of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Ziwei WangDepartment of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Heedong JeonDepartment of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada.
Akitoshi HaraDepartment of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Yoichi TakamiDepartment of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan. Electronic address: takami@geriat.med.osaka-u.ac.jp.
Koichi YamamotoDepartment of Geriatric and General Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol to visualize and quantify β-arrestin-dependent signaling of the angiotensin II type 1 receptor (AT1R). We describe steps for detecting receptor-β-arrestin interactions during early trafficking events under our imaging conditions using an afCFP-Venus FRET pair. We then detail procedures for visualizing early intracellular signaling dynamics following AT1R activation and for analyzing GPCR trafficking and signaling dynamics in living cells.

Indexed as

Cell-based AssaysCell BiologyCell MembraneSingle Cell

Identifiers

PMID42384491
PMCPMC13343132

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.