Evidence map›Paper›PMID 42395535›Full record

ArticlebioRxiv : the preprint server for biology2026

The E525K β-Myosin Mutation Causes Hypocontractility in Cardiomyocytes Without Altering Loaded Crossbridge Cycling.

Kalen Z Robeson, Timothy S McMillen, Kristina Kooiker, Kerry Y Kao, Kieran Fruebis, Travis C Tune, Michael A Geeves, Andrew P Wescott, Rachelle Soriano, Alex J Goldstein and 10 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Kalen Z RobesonDepartment of Bioengineering, University of Washington, Seattle, WA, USA.ORCID 0000-0003-1577-8280
Timothy S McMillenCenter for Translational Muscle Research, University of Washington, Seattle, WA, USA.
Kristina KooikerCenter for Translational Muscle Research, University of Washington, Seattle, WA, USA.
Kerry Y KaoCenter for Translational Muscle Research, University of Washington, Seattle, WA, USA.
Kieran FruebisDepartment of Bioengineering, University of Washington, Seattle, WA, USA.
Travis C TuneDepartment of Bioengineering, University of Washington, Seattle, WA, USA.
Michael A GeevesSchool of Biosciences, University of Kent, Canterbury, United Kingdom.
Andrew P WescottDivision of Cardiology, Medicine, University of Washington, Seattle Washington, USA.
Rachelle SorianoInstitute For Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA.
Alex J GoldsteinCenter for Translational Muscle Research, University of Washington, Seattle, WA, USA.
Matthew C ChildersDepartment of Bioengineering, University of Washington, Seattle, WA, USA.
Rama Reddy GoluguriDepartment of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA.
Divya PathakDepartment of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA.
Nathan J SniadeckiCenter for Translational Muscle Research, University of Washington, Seattle, WA, USA.
Joseph D PowersCenter for Translational Muscle Research, University of Washington, Seattle, WA, USA.
Jennifer DavisDepartment of Bioengineering, University of Washington, Seattle, WA, USA.
Farid Moussavi-HaramiCenter for Translational Muscle Research, University of Washington, Seattle, WA, USA.
James A SpudichDepartment of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA.
Kathleen M RuppelDepartment of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA.
Michael RegnierDepartment of Bioengineering, University of Washington, Seattle, WA, USA.

Funding

From proteins to cells to tissues: A multi-scale assessment of biomechanical regulation by the myosin molecular motorRM1GM131981 · NIGMS · STANFORD UNIVERSITY · PI BERNSTEIN, DANIEL, MACK, DAVID LEE · 2019 to 2023
$10.3M
UW Center for Translational Muscle Research (Overall Application)P30AR074990 · NIAMS · UNIVERSITY OF WASHINGTON · PI Jennifer Michelle Davis, DANIEL RAFTERY · 2019 to 2026
$7.5M
Integrating Transcriptome Reprogramming Into Cardiac Plasticity Regulatory MechanismsR01HL141187 · NHLBI · UNIVERSITY OF WASHINGTON · PI Jennifer Michelle Davis · 2018 to 2026
$4.0M
Uncovering The Mechanogenomic Basis For Cardiac PlasticityR01HL142624 · NHLBI · UNIVERSITY OF WASHINGTON · PI Jennifer Michelle Davis · 2018 to 2026
$3.8M
Undergraduate Research Training for Student Enhancement (U-RISE) at Cal State LAT34GM145503 · NIGMS · CALIFORNIA STATE UNIVERSITY LOS ANGELES · PI KRISHNA L FOSTER · 2022 to 2026
$3.7M
Mechanisms underlying reversal of cardiac aging by urolithin a treatmentR01AG081395 · NIA · UNIVERSITY OF WASHINGTON · PI David J. Marcinek, MICHAEL REGNIER · 2024 to 2026
$3.2M
Regulators of Myofibroblast State Stability & Fibrotic Responsiveness of the HeartR01HL162229 · NHLBI · UNIVERSITY OF WASHINGTON · PI DAVIS, JENNIFER MICHELLE · 2022 to 2025
$2.5M
Computational and Experimental Models of Myosin VariantsR01HL179584 · NHLBI · UNIVERSITY OF WASHINGTON · PI Farid Moussavi-Harami, MICHAEL REGNIER · 2026 to 2026
$1.8M
Modeling intra- and inter-cellular networks for TRPC6-dependent cardiac fibrosisR01HL176700 · NHLBI · UNIVERSITY OF VIRGINIA · PI Jennifer Michelle Davis, Jeffrey J. Saucerman · 2025 to 2026
$1.4M
Bioengineering Cardiovascular Training Grant (BCTG)T32EB032787 · NIBIB · UNIVERSITY OF WASHINGTON · PI MICHAEL REGNIER, Ying Zheng · 2022 to 2026
$969k
Dysregulated mechanosignaling in dilated cardiomyopathy caused by defective Filamin CR00HL159224 · NHLBI · UNIVERSITY OF WASHINGTON · PI POWERS, JOSEPH D. · 2023 to 2025
$747k
Single-cell proteome heterogeneity due to MYH HCM mutations and recovery by MavacamtenR01HL180748 · NHLBI · CEDARS-SINAI MEDICAL CENTER · PI MICHAEL REGNIER, Jennifer E Van Eyk · 2026 to 2026
$741k
NHLBI NIH HHS R00 HL159224NHLBI NIH HHS R01 HL141187NHLBI NIH HHS R01 HL142624NHLBI NIH HHS R01 HL162229NHLBI NIH HHS R01 HL176700NHLBI NIH HHS R01 HL179584NHLBI NIH HHS R01 HL180748NIAMS NIH HHS P30 AR074990NIA NIH HHS R01 AG081395NIBIB NIH HHS T32 EB032787NIGMS NIH HHS RM1 GM131981NIGMS NIH HHS T34 GM145503
6 · The paper itself

Abstract

The cardiac β-myosin (MYH7) mutation E525K was identified in 2012 in a patient with dilated cardiomyopathy (DCM). Work using engineered constructs has shown that this mutation stabilizes the interacting heads motif (IHM) of myosin and increases the ATPase activity of mutant motor S1 heads. However, no measurements have been made in myofilaments or cardiomyocytes to determine its effect on contractile function. Here, we present force and contractile kinetics measurements from induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) engineered for heterozygous expression of E525K. Single-cell contraction for E525K hiPSC-CMs decreased by 65%, and isometric twitch force in engineered heart tissues (EHTs) decreased by 39%. In contrast, maximal Ca

Identifiers

PMID42395535
PMCPMC13320758

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.