Evidence mapPaperPMID 42400651Full record

ArticleAnalytical and bioanalytical chemistry2026

Whole-body mass spectrometry imaging reveals metabolome and lipid peroxidation heterogeneity in zebrafish xenografts of esophageal squamous cell carcinoma.

Jiangshan Zhou, Shanyuan Zhang, Mengfan Liu, Zhonghua Wang, Zhi Zhou

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Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

5 authors.

Jiangshan ZhouKey Laboratory of Mass Spectrometry Imaging and Metabolomics (Minzu University of China), National Ethnic Affairs Commission, Beijing, 100081, China.
Shanyuan ZhangKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Thoracic Surgery II, Peking University Cancer Hospital & Institute, Beijing, 100142, China.
Mengfan LiuKey Laboratory of Mass Spectrometry Imaging and Metabolomics (Minzu University of China), National Ethnic Affairs Commission, Beijing, 100081, China.
Zhonghua WangKey Laboratory of Mass Spectrometry Imaging and Metabolomics (Minzu University of China), National Ethnic Affairs Commission, Beijing, 100081, China.
Zhi ZhouKey Laboratory of Mass Spectrometry Imaging and Metabolomics (Minzu University of China), National Ethnic Affairs Commission, Beijing, 100081, China. zhouzhi@muc.edu.cn.ORCID http://orcid.org/0009-0003-7059-2582

Funding

National Natural Science Foundation of China 21927808National Natural Science Foundation of China 82003714
6 · The paper itself

Abstract

Esophageal squamous cell carcinoma (ESCC) is a highly prevalent malignancy in China. Understanding its metabolic heterogeneity is of great significance for improving the precision of diagnosis and treatment. This study innovatively employed an adult zebrafish xenograft model, combining the spatial metabolomics and in situ derivatization method for fatty acid peroxidation products (FAPPs) based on air flow-assisted desorption electrospray ionization-mass spectrometry imaging (AFADESI-MSI) technology, to elucidate the metabolic patterns associated with ESCC and its differentiation grade. Spatial metabolomic data demonstrated that abundance alterations of glycerophospholipids and acetylated amino acids were statistically correlated with ESCC tumor formation. Levels of lysophospholipids and arachidonic acid-associated metabolites tended to increase with the progressive malignant differentiation of tumor lesions. Profiling of FAPPs showed elevated short- and medium-chain species accompanied by decreased long-chain counterparts in tumor-bearing zebrafish, which may be linked to tumor-initiated alterations in oxidative stress. Specifically, short- and medium-chain FAPPs were enriched in well-differentiated ESCC tissues, while long-chain FAPPs predominated in poorly differentiated tumors, suggestive of disparate lipid peroxidation patterns in ESCC cells at different differentiation stages. Furthermore, distinct signature metabolites were found to be specifically accumulated in the brain providing experimental clues that ESCC may remotely disturb the central nervous system metabolism of host animals. This research uncovers both tumor-induced and differentiation-dependent metabolic heterogeneity of ESCC, and validates whole-body MSI as a transformative approach to unravel tumor-host metabolic interactions.

Indexed as

Carcinoma, Squamous CellEsophageal NeoplasmsEsophageal Squamous Cell CarcinomaLipid PeroxidationMetabolomeAnimalsHeterograftsHumansMetabolomicsSpectrometry, Mass, Electrospray IonizationZebrafishAdult zebrafishEsophageal squamous cell carcinomaFatty acid peroxidation productsMass spectrometry imagingMetabolic reprogramming

Identifiers

PMID42400651

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.