ArticleApplied biochemistry and biotechnology2026
Green Synthesis of Silver Nanoparticles Using the Extract of Adenosma caeruleum R.Br.: Evaluation of Antibacterial, Antifungal and Antioxidant Activity.
Article in Applied biochemistry and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Silver nanoparticles (AgNPs) have attracted considerable attention due to their remarkable antimicrobial and antioxidant properties. In this study, AgNPs were synthesized through a green and sustainable approach using Adenosma caeruleum R.Br. extract as both reducing and stabilizing agents. The effects of key synthesis parameters, including AgNO₃ concentration, extract to precursor ratio, temperature, pH, and reaction time, were systematically optimized to achieve efficient nanoparticle formation. Comprehensive characterization using UV-Vis, FT-IR, XRD, DLS, zeta potential, TEM, and EDS analyses confirmed the successful synthesis of predominantly spherical and crystalline AgNPs with particle sizes mainly ranging from 20 to 50 nm and a Zaverage hydrodynamic diameter of 47.5 ± 1.4 nm. Despite a relatively broad particle size distribution, the nanoparticles exhibited good colloidal stability, as evidenced by a highly negative zeta potential (- 75.2 ± 1.3 mV). The biosynthesized AgNPs demonstrated notable antioxidant activity against ABTS radicals, with an IC₅₀ value of 49.8 ± 0.14 µg/mL. In addition, significant antimicrobial activity was observed against both bacterial and fungal strains. At a dose of 30 µL, inhibition zones of 21.0 ± 0.18 mm (Escherichia coli), 14.0 ± 0.13 mm (Bacillus cereus), 22.0 ± 0.17 mm (Salmonella typhimurium), 14.0 ± 0.19 mm (Staphylococcus aureus), and 12.0 ± 0.12 mm (Candida albicans) were recorded, with Gram-negative bacteria generally exhibiting greater susceptibility. The minimum inhibitory concentration (MIC) was determined to be 1.25 mg/mL for all tested microorganisms. Overall, the results demonstrate that A. caeruleum-mediated AgNPs possess antibacterial, antifungal, and antioxidant activities and highlight the potential of this indigenous medicinal plant as a sustainable resource for the green synthesis of functional nanomaterials with prospective biomedical and environmental applications.
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