Evidence mapPaperPMID 42412245Full record

ArticleBiotechnology letters2026

Insight of Pichia kudriavzevii for single cell protein production and fermentation into ethanol using Thai banana as an alternative feedstock.

Wittaya Tawong, Kawee Sujipuli, Thanita Boonsrangsom, Kumrop Ratanasut, Anurak Khieokhajonkhet, Woraphan Khongnapha, Aphitchaya Rinrit, Pongsanat Pongcharoen

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Article in Biotechnology letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Wittaya TawongDepartment of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, 65000, Thailand.
Kawee SujipuliDepartment of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, 65000, Thailand.
Thanita BoonsrangsomDepartment of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, 65000, Thailand.
Kumrop RatanasutDepartment of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, 65000, Thailand.
Anurak KhieokhajonkhetDepartment of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, 65000, Thailand.
Woraphan KhongnaphaDepartment of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, 65000, Thailand.
Aphitchaya RinritDepartment of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, 65000, Thailand.
Pongsanat PongcharoenDepartment of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, 65000, Thailand. pongsanatp@nu.ac.th.ORCID http://orcid.org/0000-0002-3836-6468

Funding

Naresuan University R2569B054
6 · The paper itself

Abstract

purposesThe objective of this study was to assess the potential of yeast Pichia kudriavzevii (NUNS-4, NUNS-5, NUNS-6, and TISTR5147) and Saccharomyces cerevisiae TISTR5606 strain to utilize banana, Musa ABB cv. (Kluai Namwa) and Musa sapientum L. cv. (Kluai Khai), biomass through the co-production of single cell protein and ethanol.

resultsAll tested strains grew well on both YPD and banana-based agar, confirming that banana residues can sustain yeast growth. Supplementation with banana biomass significantly enhanced protein and carbohydrate content. Notably, P. kudriavzevii TISTR5147, P. kudriavzevii NUNS-6, and S. cerevisiae TISTR5606 exhibited the highest single cell protein levels (50.85 ± 1.73%, 49.13 ± 1.50%, and 46.96 ± 1.07%, respectively) using Kluai Namwa, compared to controls without banana substrate. Ethanol yields were also increased when banana biomass was included. Co-fermentation of ripe pulp Kluai Khai with YPD resulted in the highest ethanol yields, reaching 77.63 ± 3.11 g/L for P. kudriavzevii NUNS-6, followed by 76.57 ± 4.49 g/L for NUNS-5, 74.17 ± 2.21 g/L for NUNS-4, and 72.32 ± 1.63 g/L for TISTR5147. Therefore, P. kudriavzevii NUNS-6 demonstrated superior efficiency as a dual producer of single-cell protein and ethanol.

conclusionThese findings demonstrate that banana biomass is a cost-effective and sustainable carbon source for single-cell protein and ethanol production. Moreover, the P. kudriavzevii NUNS strains exhibited strong potential for bioconversion of banana residues into high-value products, supporting their applicability in future large-scale biotechnological processes.

Indexed as

EthanolFungal ProteinsMusaPichiaBiomassDietary ProteinsFermentationSaccharomyces cerevisiaeThailandDietary ProteinsEthanolFungal Proteinssingle cell proteinsAgricultural wasteBanana biomassEthanol productionPichia kudriavzeviiSingle–cell protein

Identifiers

PMID42412245

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.