ArticleBio-protocol2026
An Optimized Protocol for the Characterization of Zebrafish ApoB-Containing Lipoproteins Using the LipoGlo System.
Article in Bio-protocol, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Apolipoprotein B-containing lipoproteins (ApoB-LPs) transport lipids throughout the circulation and are closely associated with cardiovascular disease in humans. Many aspects of ApoB-LP biology remain elusive, often due to their indirect characterization through the measurement of plasma triglycerides and cholesterol. The conventional approach provides limited information on ApoB-LPs number and size distribution, essential features that influence cardiovascular disease risk. Additionally, drug studies have historically been limited to the use of mammalian research models, which are not suited for high-throughput experiments. Therefore, we generated a reporter system (LipoGlo) utilizing a luciferase enzyme (NanoLuc) fused to the C-terminus of the zebrafish (
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