Evidence map›Paper›PMID 42427684›Full record

ArticlebioRxiv : the preprint server for biology2026

The mechanotransduction channel Piezo2 refines axonal projections to the accessory optic system and regulates the optokinetic reflex.

Natalie R Hamilton, Victoria N Neckles, Timour Al-Khindi, Nitya Donthi, Shun Mizutori, Renata Fu, James K Kiraly, Bea C Winship, Alex L Kolodkin, Karina Chaudhari

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Natalie R HamiltonSolomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Victoria N NecklesSolomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Timour Al-KhindiSolomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Nitya DonthiSolomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Shun MizutoriSolomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Renata FuSolomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
James K KiralySolomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Bea C WinshipSolomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Alex L KolodkinSolomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.ORCID 0000-0001-7562-5513
Karina ChaudhariSolomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.ORCID 0000-0003-3533-3027

Funding

Molecular mechanisms underlying direction-selective circuit assembly and function in the mouse visual systemR01EY032095 · NEI · JOHNS HOPKINS UNIVERSITY · PI KOLODKIN, ALEX L · 2021 to 2025
$2.3M
NEI NIH HHS R01 EY032095
6 · The paper itself

Abstract

The optokinetic reflex (OKR) is an evolutionarily conserved reflexive behavior that ensures image stabilization on the retina during global motion. It consists of smooth eye tracking movements in the direction of the moving stimulus interspersed with rapid resetting saccades. This reflex is driven by retinal ON direction-selective ganglion cells (oDSGCs), which comprise distinct subtypes tuned either to vertical or horizontal motion. oDSGCs convey directional signals to the brain via precise axonal projections to specific accessory optic system (AOS) nuclei. However, the mechanisms that establish and maintain the specificity of these circuits remain poorly understood. Here, we identify a critical role for the mechanosensitive ion channel Piezo2 in refining AOS circuitry to ensure appropriate eye movement responses. Single-cell transcriptomic profiling revealed selective enrichment of Piezo2 in horizontally-tuned oDSGCs. We show that both loss and hyperactivation of Piezo2 in retinal neurons leads to cross-coupling of horizontal and vertical OKR responses, producing aberrant diagonal eye tracking movements during horizontal optokinetic stimulation. Mechanistically, Piezo2 regulates the developmental refinement of oDSGC axonal projections within the AOS, and disruption of this process results in persistent ectopic innervation that enables aberrant crosstalk between horizontal and vertical motion pathways. These findings reveal a channel activity-dependent mechanism that ensures the functional segregation of directional motion circuits underlying gaze stabilization.

Identifiers

PMID42427684
PMCPMC13345156

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.