Evidence map›Paper›PMID 42439133›Full record

ArticleJournal of the American Chemical Society2026

Distinct, Phase-Dependent Orientations of GPI and GPI-Anchored Proteins in Lipid Bilayers Revealed Using Spin Labeling and Power Saturation Electron Paramagnetic Resonance Depth Profiling.

Md Shamiul Islam, Xin Yan, Jiatong Guo, Rajendra Rohokale, Gail E Fanucci, Zhongwu Guo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Chemical Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Md Shamiul IslamDepartment of Chemistry, University of Florida, Gainesville, Florida 32611, United States.
Xin YanDepartment of Chemistry, University of Florida, Gainesville, Florida 32611, United States.ORCID 0000-0003-1846-3241
Jiatong GuoDepartment of Chemistry, University of Florida, Gainesville, Florida 32611, United States.
Rajendra RohokaleDepartment of Chemistry, University of Florida, Gainesville, Florida 32611, United States.ORCID 0000-0001-8083-0466
Gail E FanucciDepartment of Chemistry, University of Florida, Gainesville, Florida 32611, United States.ORCID 0000-0003-1960-160X
Zhongwu GuoDepartment of Chemistry, University of Florida, Gainesville, Florida 32611, United States.ORCID 0000-0001-5302-6456

Funding

Synthetic and Biological Studies of GPI Conjugates and GPI Anchorage to Cell MembranesR35GM131686 · NIGMS · UNIVERSITY OF FLORIDA · PI Zhongwu Guo · 2019 to 2026
$2.5M
Upgrade to E500 X- and Q-Band CW EPR Spectrometer for Biomedical ResearchS10RR031603 · NCRR · UNIVERSITY OF FLORIDA · PI FANUCCI, GAIL E · 2011 to 2011
$523k
NCRR NIH HHS S10 RR031603NIGMS NIH HHS R35 GM131686
6 · The paper itself

Abstract

Glycosylphosphatidylinositols (GPIs) help anchor and organize surface proteins, while the interfacial conformations of GPIs and GPI-anchored proteins (GPI-APs) on cells, which determine their functions, remain unclear. In this work, a bifunctional GPI anchor bearing clickable azide and biotin─an affinity tag─was used to access labeled GPI anchors and GPI-AP analogs. Employing spin-labeled GPI and GPI-streptavidin conjugate as probes, together with spin-labeled "ruler" lipids and power saturation electron paramagnetic resonance (EPR) profiling, we quantified their accessibilities to membrane-tethered and soluble spin relaxants, with the probes and rulers incorporated in 1-palmitoyl-2-oleoyl-

Indexed as

GlycosylphosphatidylinositolsLipid Bilayers1,2-DipalmitoylphosphatidylcholineElectron Spin Resonance SpectroscopyPhosphatidylcholinesSpin LabelsStreptavidin1,2-Dipalmitoylphosphatidylcholine1-palmitoyl-2-oleoylphosphatidylcholineGlycosylphosphatidylinositolsLipid BilayersPhosphatidylcholinesSpin LabelsStreptavidin

Identifiers

PMID42439133
PMCPMC13405882

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.