Evidence map›Paper›PMID 42444546›Full record

ArticleAddiction biology2026

Transcriptional Response to Chronic Long-Access Fentanyl Self-Administration in Rat Habenula and Amygdala.

Robin Magnard, Daianna Gonzalez-Padilla, Ege A Yalcinbas, Emma Chaloux-Pinette, Nicholas J Eagles, Michael S Totty, Patricia H Janak, Leonardo Collado-Torres, Kristen R Maynard

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Addiction biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Robin MagnardDepartment of Psychological and Brain Sciences, Krieger School of Arts and Sciences, Johns Hopkins University, Baltimore, Maryland, USA.ORCID https://orcid.org/0000-0002-7818-9654
Daianna Gonzalez-PadillaLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.ORCID https://orcid.org/0009-0005-8348-3195
Ege A YalcinbasLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.
Emma Chaloux-PinetteThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
Nicholas J EaglesLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.
Michael S TottyDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA.
Patricia H JanakDepartment of Psychological and Brain Sciences, Krieger School of Arts and Sciences, Johns Hopkins University, Baltimore, Maryland, USA.
Leonardo Collado-TorresLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.ORCID https://orcid.org/0000-0003-2140-308X
Kristen R MaynardLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, USA.

Funding

INTERDISCIPLINARY TRAINING PROGRAM IN NEUROSCIENCEST32MH015330 · NIMH · JOHNS HOPKINS UNIVERSITY · PI MARGOLIS, RUSSELL L · 1985 to 2024
$6.2M
Optogenetic Testing of Mechanims of Dopamine Action in Pavlovian LearningR01DA035943 · NIDA · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JANAK, PATRICIA H. · 2014 to 2023
$4.1M
Integrated single cell and spatial mapping of habenula circuitry to identify projection-specific molecular pathways associated with opioid exposure.R21DA060407 · NIDA · LIEBER INSTITUTE, INC. · PI MAYNARD, KRISTEN ROSE · 2024 to 2025
$506k
Lieber Institute for Brain DevelopmentNIDA NIH HHS R01 DA035943NIDA NIH HHS R21 DA060407NIH HHS R01DA035943NIH HHS R21DA060407NIH HHS T32MH015330NIMH NIH HHS T32 MH015330
6 · The paper itself

Abstract

Fentanyl is a potent synthetic opioid associated with overdose. However, little is known about fentanyl-induced molecular adaptations in the habenula and amygdala, two brain regions implicated in opioid use and withdrawal. We performed bulk RNA-sequencing in the rat habenula and amygdala to identify transcriptomic changes associated with fentanyl intake. Male rats self-administered intravenous saline or fentanyl over 22-24 days. Ninety minutes following the final session, brains were collected for transcriptomic profiling. In Hb, we identified 453 differentially expressed genes (DEGs) between saline and fentanyl rats, with upregulated genes associated with synaptic transmission and ionic conductance. In the amygdala, we identified 3041 fentanyl-associated DEGs with upregulated genes implicated in metabolic and vesicular functions. Downregulated genes in both regions were enriched for extracellular matrix functions. Integration of DEGs with single-cell RNA-sequencing data from rodents and humans revealed that fentanyl DEGs were enriched in specific habenula and amygdala cell type markers. Furthermore, fentanyl downregulated DEGs in the amygdala were enriched in genes associated with the risk for substance use disorders. Together, we define how fentanyl intake alters transcriptional programs in the rat habenula and amygdala, and we link these changes to specific human cell types and risk genes for neuropsychiatric disorders and addiction.

Indexed as

AmygdalaAnalgesics, OpioidFentanylHabenulaTranscriptomeAnimalsGene Expression ProfilingMaleRatsRats, Sprague-DawleySelf AdministrationAnalgesics, OpioidFentanyl

Identifiers

PMID42444546
PMCPMC13366401

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.