Evidence map›Paper›PMID 42446569›Full record

ArticleNaunyn-Schmiedeberg's archives of pharmacology2026

A combination of Nrf-2 activator, kinetin and zinc provides neuroprotection through NLRP3-NF-ԟB signaling in traumatic brain injury.

Shivani Agarwal, Mohd Shahrukh, Divya Vohora, Suhel Parvez, Abul Kalam Najmi, Mohd Akhtar

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Article in Naunyn-Schmiedeberg's archives of pharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

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6 authors.

Shivani AgarwalDepartment of Pharmacology, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India, 110062.
Mohd ShahrukhDepartment of Medical Elementology and Toxicology, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India, 110062.
Divya VohoraDepartment of Pharmacology, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India, 110062.
Suhel ParvezDepartment of Medical Elementology and Toxicology, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India, 110062.
Abul Kalam NajmiDepartment of Pharmacology, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India, 110062.
Mohd AkhtarDepartment of Pharmacology, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India, 110062. makhtar_ph@jamiahamdard.ac.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nuclear erythroid 2-related factor (Nrf-2) activators have been used in previous research in inhibiting the multifaceted pathophysiology of traumatic brain injury (TBI). Moreover, combinatorial treatment strategies provide an additive pharmacological effect when compared with single treatment against brain injury. Therefore, the current study aimed to evaluate the neuroprotective effect of a Nrf-2 activator, kinetin (plant cytokinin, 6-Furfurylaminopurine) and zinc along with their combination on pre-clinical TBI. The acute injury was induced in male Wistar rats by controlled cortical impact and kinetin (5 mg/kg and 10 mg/kg) along with zinc picolinate (6 mg/kg) was administered. Several neurobehavioral tests like open field, beam balance and grip strength were performed post injury. Biochemical, histopathological and immunohistochemical analysis were also carried out. The expression of NLRP3-NF-ԟB pathway proteins were evaluated using techniques like ELISA and immunohistochemistry. Histopathological analysis was performed through H & E staining method. The results showed less exploration and grip strength in injured rats which was improved after treatment with kinetin. Injured rats showed a slight increase in brain and serum Nrf-2 levels which was profoundly increased by treatment. We also found an increase in brain and serum levels of an antioxidant defence system and a decrease in levels of inflammatory cytokines after treatment with both kinetin and zinc picolinate. There was a significant decrease in levels of NF-ԟB and NLRP3 inflammasome. Further, histopathological and immunohistochemistry analysis revealed neuroprotective effect of both the compounds on TBI. We can conclude that kinetin and zinc, alone and their combination show neuroprotective effects in TBI by activating Nrf-2 anti-oxidant defence and inhibiting NLRP3-NF-ԟB pathways. These findings support future evaluation of plant cytokinin in combination with a bio metal in attenuating secondary injury in TBI.

Indexed as

InflammasomeKinetinNrf-2 activatorTBIZinc

Identifiers

PMID42446569

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.